Tymora Analytical Operations, LLC
table of contents
EVtrap
Here’s a better way to conduct EV (exosome) proteins and post-translational modifications (PTMs) analysis .
Tymora Analytical developed an improved approach to analyze EV (exosome) proteins, phosphoproteins and other cancer-signaling molecules.
This technology, based on non-antibody affinity beads, is EVtrap (Extracellular Vesicles Total Recovery and Purification) to help others learn more about cancer and other diseases.
Tymora Analytical routinely identifies over 1,000 unique proteins from EVs from only 0.01 mL of plasma or 0.2 mL of urine, a significant improvement over any prior data.
A Non-Invasive, Low-Cost Procedure to Detect Cancer Biomarkers from Biological Fluids
Take a look at out our outreach article published in Scientia, the leading science communication publication. In the article, Tymora Analytical discuss our non-invasive approach of isolating EVs from biofluids to discover more protein biomarkers than in previous methods. Tymora Analytical also explain how we’re growing our network of collaborators by offering our EV proteomics services to researchers like you.
Who should partner with Tymora?
Contract Research Organizations (CROs)
If you conduct research for pharmaceutical, biotechnology, or medical device companies—with us, you can deliver innovative research and help them save costs on biomarker discovery and drug development.
Pharmaceutical and Biotechnology Companies
If you work in pharmaceuticals or biotechnology, you can study biofluid samples with our non-invasive biomarker discovery to develop a companion diagnostic for your therapy.
Research Scientists and Clinical Program Directors
If your career involves researching for cures or overseeing a research team, you can partner with Tymora Analytical to gain access to more effective, state-of-the-art technologies.
Why partner with Tymora Analytical?
Whether you’re researching biomarker discovery, mass spectrometry analysis, or developing a new drug, we understand how important your work is. That’s why we’ve developed a more effective way for you to analyze and develop new disease markers.
Currently, genetic screens dominate the market. However, genes just show one’s predisposition to developing a disease— for example, a 40% chance of getting breast cancer. In addition, CTCs and cfDNA are present at very low levels and are extremely difficult to detect at early stages.
Non-invasive protein analysis, on the other hand, provides real-time information about one’s disease progression. Tymora Analytical conducts this biopsy-free test using exosomes and other EVs so we can analyze what’s actually going on.
We’ve developed new EV analysis services for biomarker identification—EVtrap—to help other researchers detect thousands of active disease proteins and phosphoproteins from biofluids like blood, urine, and saliva. And, we would love to collaborate with you for more biomarker discoveries.
When you work with us, you’re getting:
- ・Innovative R&D products and services
- ・Unprecedented technology like EVtrap
About EVtrap - ・High-resolution proteomics solutions and LC-MS/MS services
- ・Accurate information about a disease’s progression
- ・Grant assistance for funding your research
- ・Helpful resources for ongoing education
plMAGO
Phosphorylation plays a crucial role in regulating various cellular functions. Detection of phosphorylation generally requires expensive and difficult-to-obtain phosphoantibodies or radioactive ATP labeling. However, Tymora’s plMAGO enables highly efficient and safe detection of phosphorylation without the use of antibodies. Furthermore, unlike phosphoantibodies, it allows comprehensive detection of phosphorylation non-specifically to the amino acid sequence.
Characteristics of phosphorylation detection using plMAGO
- ・Highly sensitive and comprehensive detection
- ・Excellent quantitative and linear performance
- ・Can be used for in vitro kinase/phosphorylation assays
- ・Detects in vivo phosphorylated proteins after immunoprecipitation
- ・Multiplex detection
(Click to enlarge)
plMAGO consists of a water-soluble spherical nanopolymer (dendrimer) that is multifunctionalized with reactive groups such as Ti(IV) ions for highly sensitive phosphorylation detection and reporting groups such as biotin and fluorophores for enhanced detection sensitivity.
1: On-membrane detection (Western Blot & dot-blot format)
(Click to enlarge)
Kit contents：pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. (ECL substrateは含まれません)
Product Specifications：
Protocol： Product instructions
Products List
| No. | Name | Volume |
|---|---|---|
| 800 | pIMAGO HRP Detection on Western Blot (complete kit) | 10 mini-blot, 40 mini-blot |
| 801 | pIMAGO Fluor 550 Detection on Western Blot (complete kit) | 10 mini-blot, 40 mini-blot |
| 802 | pIMAGO Fluor 680 Detection on Western Blot (complete kit) | 10 mini-blot, 40 mini-blot |
| 803 | pIMAGO Fluor 800 Detection on Western Blot (complete kit) | 10 mini-blot, 40 mini-blot |
| BLOCK-WB | 10x Optimized Blocking Buffer for Western Blot | 50 ml, 200 ml |
| HRP | Avidin-HRP (Peroxidase) | 200 ul, 500 ul, 2 ml |
| FL550 | Avidin-Fluor 550 | 200 ul, 500 ul, 2 ml |
| FL680 | Avidin-Fluor 680 | 200 ul, 500 ul, 2 ml |
| FL800 | Avidin-Fluor 800 | 200 ul, 500 ul, 2 ml |
| CNTRL-WB | Control Phosphoprotein for Western Blot | |
| PMGO | Extra pIMAGO (Reagent only) | 50 ml, 200 ml |
2: On-microplate detection (ELISA format)
(Click to enlarge)
Kit contents：pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, high-binding adsorption plates and a control phosphoproteins. HRP-based kits also include colorimetry substrate and stop solution.
Product Specifications：
Protocol： Product instructions（HPR-based detection）
Product instructions（Fluor-based detection）
Products List
| No. | Name | Volume |
|---|---|---|
| 900 | pIMAGO HRP Colorimetric Detection on Microplate (complete kit) | 100 wells, 400 wells |
| 901 | pIMAGO Fluor 550 Detection on Microplate (complete kit) | 100 wells, 400 wells |
| 902 | pIMAGO Fluor 680 Detection on Microplate (complete kit) | 100 wells, 400 wells |
| 903 | pIMAGO Fluor 800 Detection on Microplate (complete kit) | 100 wells, 400 wells |
| HRP | Avidin-HRP (Peroxidase) | 200 ul, 500 ul, 2 ml |
| FL550 | Avidin-Fluor 550 | 200 ul, 500 ul, 2 ml |
| FL680 | Avidin-Fluor 680 | 200 ul, 500 ul, 2 ml |
| FL800 | Avidin-Fluor 800 | 200 ul, 500 ul, 2 ml |
| CNTRL-ELISA | Control Phosphoprotein for ELISA | |
| PLATE | High Binding Capacity Clear 96-well Plates | 5 plats, 20 plats |
| BIND | On-plate Protein Binding Buffer | 50 ml, 200 ml |
| BLOCK-PL | Optimized Blocking Buffer for Microplate | 50 ml, 250 ml |
| PMGO | Extra pIMAGO (Reagent only) | 50 ml, 200 ml |
3: Microarray detection
plMAGO (peptide/protein arrays, kinase substrate arrays and antibody arrays) for microarrays is currently under development. If you are interested in this product or would like to collaborate, please contact Tokyo Future Style.
(Click to enlarge)
PolyMAC
Enrichment of phosphopeptides is a crucial step in phosphoproteomics research. Tymora’s PolyMAC (Polymer-based Metal-ion Affinity Capture) enables efficient, sensitive, and accurate enrichment of phosphopeptides. PolyMAC consists of a soluble nanopolymer with a metal ion to chelate phosphopeptides in solution.
(Click to enlarge)
Kit contents：PolyMAC-Ti reagent, capture beads, loading buffer, washing buffer, capture buffer and elution buffer. Agarose capture-based kits also include re-usable spin columns.
Protocol： Product Specifications（magnetic beads capture）
Product Specifications（agarose beads capture）
Products List
| No. | Name | Volume |
|---|---|---|
| 701 | PolyMAC-Ti Agarose Phosphopeptide Enrichment Kit | 50, 100 enrichments |
| 700 | PolyMAC-Ti Magnetic Phosphopeptide Enrichment Kit (recommended) | 50, 100 enrichments |
| SPIN | Extra Spin Columns for PolyMAC Agarose capture | 10, 100 colums |
| MAGB | Magnetic Capture Beads | 2.5 ml, 5 ml |
| AGARB | Agarose Capture Beads | 2.5 ml, 5 ml |
| 702 | Agarose Capture Beads | 500 ul, 1 ml |
Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL：029-851-9222 FAX：029-851-9220
