PRIMACYT – Rainbow trout liver S9 subcellular fractions
Our Strengths
PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes.
In 2008, we have received the Research Prize of the German Ministry of Nutrition, Agriculture and Consumer Protection for the “Development and validation of a serum-free, standardized and re-usable human hepatocyte culture system for the analysis of food, drugs and chemicals”.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as a member of the laboratory network responsible for the method validation.
Main products, liver-derived and skin-derived products, are cryopreserved at the first passage stage, and the product information below clearly states the viability, recovery rate, suitable plates for culture, and test results for 3D culture.
Rainbow trout (Oncorhynchus mykiss) liver subcellular fractions
Order Information
Overview
Pooled Fish S9 fractions – a new in-vitro model System.
Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.
The OECD Test Guideline 319B, Determination of in vitro intrinsic clearance using cryopreserved rainbow trout liver S9 sub-cellular fractions (RT-S9), was released on June 27, 2018. PRIMACYT now offers cryopreserved pools of rainbow trout liver S9 fractions prepared and characterized in accordance with this guideline.
Literature
Rainbow Trout – a new in-vitro System
More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies.
Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016).
These and other supporting research results lead to the implementation of OECD Test Guidelines 319A and 319B on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.
Additional Literature
Tust M., Kohler M., Lagojda A, and Lamshoeft M: Comparison of the in vitro assays to investigate the hepatic metabolism of seven pesticides in Cyprinus carpio and Oncorhynchus mykiss.
ntra- and interlaboratory reliability of a cryopreserved trout hepatocyte assay for the prediction of chemical bioaccumulation potential.
Hepatocytes as in vitro test system to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species.
Reliability of In Vitro Methods used to Measure Intrinsic Clearance of Hydrophobic Organic Chemicals by Rainbow Trout: Results of an International Ring Trial.
Product List
| Product Code | Lot | Controlled housing conditions | Sex | Inventory (vials) | Specification | Characterisation | Protein (mg/mL) | Unit Size (mL) |
|---|---|---|---|---|---|---|---|---|
| FL-MIC-2P10 | FL170613 | no | female | 24 in stock | Microsomes, Pool of 10 | Phase I, SDS PAGE | 10 | 1 |
| FL-S9-2P10 | FL170613 | no | female | 43 in stock | S9, Pool of 10 | SDS PAGE | 10 | 1 |
| RTL-S9-3P8 | RTL230704 | yes | mixed | 55 in stock | S9, Pool of 8 | Phase I+II | 20 | 0.5 |
| RTL-S9-2P10 | RTL230209 | yes | female | 438 in stock | S9, Pool of 10 | Phase I and II metabolism, SDS Page | 20 | 0.5 |
Please contact us for any inquiries, questions, or information requests.
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