PRIMACYT – Chicken liver subcellular fractions
Our Strengths
PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes.
In 2008, we have received the Research Prize of the German Ministry of Nutrition, Agriculture and Consumer Protection for the “Development and validation of a serum-free, standardized and re-usable human hepatocyte culture system for the analysis of food, drugs and chemicals”.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as a member of the laboratory network responsible for the method validation.
Main products, liver-derived and skin-derived products, are cryopreserved at the first passage stage, and the product information below clearly states the viability, recovery rate, suitable plates for culture, and test results for 3D culture.
Chicken (Gallus gallus domesticus) liver subcellular fractions
Order Information
Overview
Animal health plays an important role not only for the welfare of pets and domestic farm animals but also for animals used in food production. For the development of veterinary drugs we offer liver derived products from a variety of species including dog, horse, sheep, goat, pig, poultry and fish.
A number of these animals also play an important role in environmental risk assessment of herbicides and fungicides used in crop protection. Isolated hepatocytes and liver subcellular fractions from ruminants, poultry and fish have increasingly become an important research tool in metabolism and bioaccumulation studies. Cost-saving test methods using hepatocyte cultures and subcellular fractions as alternatives to animal experiments will reduce the number of animal experiments and are currently introduced into OECD test guidelines.
Literature
Toxicologist are using liver fractions like microsomes from chicken and other birds as model systems to study in vivo metabolism, bioaccumulation and the effects of agrochemicals and fungicides on biotransformation since more than 40 years. For example, turkey liver subcellular fractions have been use to analyze metabolism, biotransformation and toxic effects of, e.g., mycotoxins, pharmaceuticals, and antibiotics
Developing regulatory activities (e.g., REACh) require bioaccumulation to be assessed for thousands of chemicals. On top of this, there is increasing pressure to reduce, refine and replace animal tests. This leads to an urgent need for the evaluation of in vitro systems to provide significant data for the estimation of bioaccumulation of chemicals of all kind in human and animals.
Murcia H, Cruz A, Leon J, Gonzales-Curbelo MA: Study on the inhibitory effect of furafylline and troleandomycin in the 7-methoxyresorufin-O-demethylase and nifedipine oxidase activities in hepatic microsomes from four poultry species using high-performance liquid chromatography coupled with fluorescence and ultraviolet detection. J Pharm Biomed Anal, 2019, 164:148-154
Murcia HW, Díaz GJ, Cepeda SM: Enzymatic activity in turkey, duck, quail and chicken liver microsomes against four human cytochrome P450 prototype substrates and aflatoxin B1. J Xenobiotics, 2011, 1:e4: 17-21
Product List
| Product Code | Lot | Sex | Inventory (vials) | Specification | Characterisation | Protein (mg/mL) | Unit Size (mL) |
|---|---|---|---|---|---|---|---|
| PL-S9-201 | PL160106-1 | female | 76 in stock | S9, single donor | Phase I, SDS PAGE | 5 | 1 |
| PL-MIC-201 | PL160106-1 | female | 37 in stock | microsomes, single donor | Phase I, SDS PAGE | 5 | 1 |
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