PRIMACYT – Common Carp liver S9 subcellular fractions|Tokyo Future Style, Inc.

PRIMACYT – Common Carp liver S9 subcellular fractions

We develop and provide cell culture systems for the prediction of toxic and long-term effects of agents on the human body.　Worldwide support of biomedical and cell biology research as well as industrial communities.We accompany projects to fully understand the metabolism, toxicity and functional effects of the liver system.

Our Strengths

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes.
In 2008, we have received the Research Prize of the German Ministry of Nutrition, Agriculture and Consumer Protection for the “Development and validation of a serum-free, standardized and re-usable human hepatocyte culture system for the analysis of food, drugs and chemicals”.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) since 2013 and serves as a member of the laboratory network responsible for the method validation.
Main products, liver-derived and skin-derived products, are cryopreserved at the first passage stage, and the product information below clearly states the viability, recovery rate, suitable plates for culture, and test results for 3D culture.

Common carp (Cyprinus carpio) liver subcellular fractions
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Overview

Pooled Fish S9 fractions – a new in-vitro model System.

Sweetwater fish like Common carp (Cyprinus carpio) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risks of chemicals, including herbicides, crop protection products, and fertilizers. Apart from Rainbow trout, Common carp is one of the best-studied fish species.

The OECD Test Guideline 319B, Determination of in vitro intrinsic clearance using cryopreserved rainbow trout liver S9 sub-cellular fractions (RT-S9), was released on June 27, 2018. PRIMACYT now offers cryopreserved pools of rainbow trout liver S9 fractions prepared and characterized in accordance with this guideline.

Literature

Tust M., Kohler M., Lagojda A., and Lamshoeft M: Comparison of the in vitro assays to investigate the hepatic metabolism of seven pesticides in Cyprinus carpio and Oncorhynchus mykiss.
https://doi.org/10.1016/j.chemosphere.2021.130254

Regnery J., Riegraf C., Jacob S., and Friesen A.: New insights on in vitro biotransformation of anticoagulant rodenticides in fish. https://doi.org/10.1016/j.chemosphere.2022.133727

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Laue H., Hostettler L., Jenner K. J., Sanders G., and Natsch A.: Bioconcentration Assessment in Fish Based on In Vitro Intrinsic Clearance: Predictivity of an Empirical Model Compared to In Vitro-In Vivo Extrapolation Models. https://doi.org./10.1021/acs.est.3c02216

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Product List

Product Code	Lot	Sex	Inventory (vials)	Specification	Characterisation	Protein (mg/mL)	Unit Size (mL)
CCL-S9-1P3	CCL200702-1	male	252 in stock	S9, Pool of 3	Phase I+II	10	0.5
CCL-S9-3P4	CCL200702-2	mixed (2 m, 2 f)	170 in stock	S9, Pool of 4	Phase I+II	10	1
CCL-S9-2P7	CCL200702-3	female	584 in stock	S9, Pool of 7	Phase I+II	10	0.5
CCL-S9-1P2	CCL190711	female	54 in stock	S9, Pool of 2	Phase I+II, SDS PAGE	20	0.5

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