Magnetic Beads – MAGic BioProcessing
MAGic BioProcessing WebsiteAPPLICATIONS
ANTIBODIESMonoclonal – and polyclonal antibodies
Monoclonal antibodies (mAbs) are the most accelerating drug products on the market. With an increasing demand of mAbs, a significant burden has been placed on the mAbs capture and downstream process. The Protein A column chromatography process, which can reach over 90% purity, is the overall golden standard process for the purification of mAbs. However, before the Protein A column capture step, the cells producing the mAbs needs to be removed by filtration or centrifugation in order to prevent column clogging issues. This step will decrease the overall yield, is costly, can take many hours, and also to increase host cell protein (HCP) levels and proteolytic activity in the clarified material, due to cell lysis demanding following challenging purification steps.
Our MAGic methodology is a one-step batch separation product-recovery process, directly applied on the cell broth, and provides increased product yield and savings in terms of time, material, and other resources, while also reducing the HCP levels. The methodology includes a magnetized resin, our MAGicBeads dedicated for mAb capture, together with specially designed magnetic separators, our MAGicSep units, optimized for efficient separation of the magnetized resin. Our products gives unique scalability for the lab, pilot and the process scale.
Our products dedicated for antibodies
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MAGicBeads mAb
MAGicBeads mAb is a magnetic bead coupled with an alkali resistant protein A ligand optimized for purification of mAbs directly from the cell broth. The high bindning capacity, stability of the matrix and alkali resistance of the ligand makes the resin suitable for repeated purification cycles without loosing performance when cleaning with 0.1-0.2M NaOH between the runs.
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MAGicBeads PrtA
MAGicBeads PrtA is an agarose-based magnetic bead coupled with an recombinant protein A ligand for capture and recovery of monoclonal and polyclonal antibodies directly from complex feedstocks.
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MAGicBeads SerA
MAGicBeads SerA are optimized for the purification of polyclonal antibodies from serum and monoclonal antibodies from ascites fluid samples. The magnetic beads have a binding capacity of 60 mg human or rabbit IgG per ml settled beads.
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MAGicSep Lab, Pilot and Process
The MAGicSep Lab and Pilot units are designed for durability with a lacquered plastic shell and nickel plated neodymium magnets placed safely inside. They fit standard test tubes and are available in sizes from 1 ml to liters. The MAGicSep Process units are made for efficient capture of 1-40 liters of MAGicBeads from thousands of litres of complex feedstocks.
CELLS
Today several projects within therapy are based on using viable cells as personalized medicines and these cells have to be pure and active. The separation and sorting processes of the cells is thus of critical nature.
Numerous cell isolation and sorting techniques have been developed for benchtop and clinical settings that are based on either physical properties of the cell or on cell-surface affinity. Standard separation techniques based on the cells physiological properties include filtration, centrifugation and sedimentation. However, in the field of cell therapy the cell size or density differences are not significant and effective cell separation is hindered. Therefore other methods are used.
Isolation techniques that are based on the cell-surfaces antigen binding such as fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) is now popular and attractive. Because of its specificity, flexibility, ease-of-use and scalability MACS is currently the technique with the greatest potential for cell separation in the cell therapy field. Until now, effective magnetic-based cell separation methodologies are present for up to 100ml sample volumes.
Our novel MAGic methodology including magnetized resins and magnetic separators for all scales of purification of cells for therapy use. The technique offers a unique scalability from the lab bench through pilot and to the final process scale.
Our products dedicated for cells
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MAGicBeads ACT
Surface activated magnetic beads for covalent couplig of antibodies. By coupling cell-surface antigen active antibodies, the magnetic beads can be used both in positive selection and in negative selection (untouched) approaches of cell separation. We offer customized covalent coupling service of antibodies to the activated beads.
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MAGicSep Lab, Pilot and Process
Our magnetic separators (MAGicSep) gives unique scaleability for the lab, pilot and the process scale.
PRODUCTS
Magnetic resins
MAGic bioprocessing provides magnetic agarose-based resins that are used to recover biologics or other biomolecules directly from crude sample mixtures eliminating the need of centrifugation or filtration. Our MAGicBeads are highly porous which provides a large available surface area (50m2/ml) resulting in exceptional high binding capacities, similar to column resins. The beads are also strongly magnetized (40emu/g) and stable due to our proprietary manufacturing method which provides consistent and fast magnetic separation even in large sample volumes (litres).
Magnetic separators
MAGic bioprocessing provides magnetic separators (MAGicSep) that are optimized for easy and efficient capture of the magnetic resin from crude sample mixtures. The standard units are designed for durability with a lacquered plastic shell and nickel plated neodymium magnets placed safely inside. They fit standard test tubes and are available in sizes from 1 ml to liters. The larger custom units are proprietary designed magnetic filters (MAGicSep Process) made for efficient capture of 1-40 liters of magnetic beads from infinite volumes of complex feedstocks.
AFFINITY BEADS
Product No.: 1500MAGicBeads mAb
Super-paramagnetic porous beads coupled with an alkali resistant protein A ligand. Optimized for purification of monoclonal antibodies directly from cell culture harvests, thus eliminating centrifugation or filtration, such as from hybridoma or CHO cells, where sanitization of beads with NaOH is desired between the uses. High bindning capacity and fast magnetic response makes the beads suitable for separation even in large-volume samples.
- Recover your mAb and skip centrifugation and filtration to remove cells
- Top performance binding capacity, over 45mg IgG/ml of settled beads
- Ability to clean the beads between the purification cycles using 0.1-0.2M NaOH
- Stable and reusable
| MAGicBeads mAb | Product No. |
|---|---|
| 1 ml beads | 1501 |
| 5 ml beads | 1502 |
| 25 ml beads | 1503 |
| 1 liter beads | 1504 |
Product No.: 1100MAGicBeads PrtA
- Super-paramagnetic porous beads coupled with recombinant protein A
- Recover your mAb and skip centrifugation and filtration to remove cells
- Top performance binding capacity, over 45mg IgG/ml of settled beads
| MAGicBeads PrtA | Product No. |
|---|---|
| 1 ml beads | 1101 |
| 5 ml beads | 1102 |
| 25 ml beads | 1103 |
| 1 liter beads | 1104 |
Product No.: 1200MAGicBeads SerA
MAGicBeads SerA are optimized for purification of antibodies from serum and ascites fluid samples and for IgG serum depletion. The beads have a binding capacity of 60 mg human or rabbit IgG per ml settled beads.
- Purification and depletion of IgG from serum and ascites
- Coupled with recombinant protein A.
- Extremely high binding capacity, over 60mg IgG/ml of beads
- Hydrophilic matrix resulting in very high specificity and low background
| MAGicBeads SerA | Product No. |
|---|---|
| 1 ml beads | 1201 |
| 5 ml beads | 1202 |
| 25 ml beads | 1203 |
| 1 liter beads | 1204 |
DATA AND APPLICATION NOTES
DOCUMENTS
ACTIVATED BEADS
Product No.: 1300MAGicBeads ACT
Surface activated magnetic agarose beads employing our patented coupling chemistry. Any ligand containing amino groups or thiols, e.g. proteins and peptides can be covalently bound to the beads using a simple process. All steps are performed at physiological conditions and room temperature, without using any hazardous reagents or organic solvents. After coupling of the ligand, the magnetic beads can be used for affinity purification and immunoprecipitation.
- Activated magnetic beads for coupling your own ligands of choice
- Simple coupling process at ambient temperature
- Suitable for coupling of ligands containing sulphydryl- or amino groups
- Gives a stable bond between bead and ligand
- High coupling capacity, 10-30mg IgG/ml beads
- Couple 20mg IgG/ml beads with a yield of 90%
| MAGicBeads ACT | Product No. |
|---|---|
| 1 ml beads | 1301 |
| 5 ml beads | 1302 |
| 25 ml beads | 1303 |
| 1 liter beads | 1304 |
DATA AND APPLICATION NOTES
DOCUMENTS
CUSTOMIZED MAGNETIC BEADS
Product No.: 1000-customMAGicBeads CUSTOM
MAGic Bioprocessing are specialized in the development and production of tailor made magnetic bead products for a variety of different applications. We are able to produce customized magnetic bead products in the scale from µl up to liter of settled beads.
The parameters that can be optimized are:
- Degree of magnetization
- Size of beads
- Concentration of agarose
- Surface-functional groups
- Ligand-coupling services
Please contact us for free preliminary discussions.
DURABLE MAGNETIC SEPARATORS FOR LAB, PILOT AND PROCESS SCALE
The MAGicSep series of laboratory magnetic separators have been optimized for use with MAGicBeads and are ideal for most bioseparation applications. The LAB and PILOT units are designed for durability with a lacquered plastic shell and nickel plated neodymium magnets placed safely inside. The separators fit standard test tubes and are available in sizes from 1 ml to 500 ml. The combination of MAGicBeads and MAGicSep is a simple biomagnetic separation platform that requires no special training to use. The separators require very little maintenance and several batches can be processed in parallel using the same unit, making the running costs very low. The larger custom units are proprietary designed magnetic separators (MAGicSep Process) made for efficient capture of 1-40 liters of magnetic beads from infinite volumes of complex feedstocks.
| MAGicSep unit | Product no. |
|---|---|
| LAB rack | 2000 |
| LAB cube | 2100 |
| PILOT 50 | 2200 |
| PILOT 500 | 2300 |
| PROCESS Compact | 3100 |
| PROCESS Maxi | 3400 |
DOCUMENTS
EXPLORE OUR SCALES OF OPERATION
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MAGic LAB
- Process volumes from 50 µl to 5 ml
- Separate from 5 µl to 200 µl of magnetic beads
- Purify up to 6 mg proteins each cycle
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MAGic PILOT
- Process volumes from 3 ml to 500 ml per purification cycle
- Separate from 10 µl up to 30 ml of magnetic beads
- Purify up to 1 g proteins each cycle
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MAGic PROCESS
- Process volumes from 10 l up to 4,000 l
- Separate from 1 l to 40 l magnetic beads
- Recover up to 1 kg of proteins each cycle
At the research lab, small-scale processing of peptides, proteins, antibodies or cells from crude sample mixtures like serum, whole blood, ascites, food-samples or unclarified cell cultures can be performed in volumes from 100 µl to 2 ml. By using 10-200 µl of MAGicBeads together with the MAGicSep LAB cube or LAB rack enables fast, reliable and easy separation of up to 6 mg of proteins per each tube via serial or parallel-process manual configuration.
Our MAGicBeads are also compatible with robotic system such as the KingFisher™ Flex unit (Thermo Fisher) allowing the use of our beads in automatic setups for high-throughput screening.
DATA AND APPLICATION NOTES
During the development phase, medium-scale processing of peptides, proteins or antibodies from clarified- or unclarified cell-culture mixtures is performed in sample volumes from 5 ml up to a few liters. The use of the MAGicSep PILOT 50 or MAGicSep PILOT 500 together with 200µl – 30ml of settled MAGicBeads offers a durable, convenient and fast separation platform of up to gram quantities of proteins per each bottle via serial or parallel-process manual configuration.
By combining the different MAGicSep units, low expressed target peptides and proteins can be captured from a large volume and then washed and eluted in a smaller sized vessel, thus achieving a significant product concentration.
DATA AND APPLICATION NOTES
During the production phase of biopharmaceuticals, large-scale recovery of the product from an unclarified cell-culture is performed in a one step clarification and purification by using the propietary designed MAGicSep PROCESS units together with the affinity-coupled MAGicBeads. The magnetic filter separators are capable to capture up to 40 liters of MAGicBeads at high flow rates making them suitable to process up to kilo grams of proteins or antibodies per purification cycle.
By utilizing the magnetic-based capture approach, the biopharmaceutical can be recovered directly from the cell-containing culture with the same or better quality as for a conventional 2-step clarification and purification mode using depth filters and column chromatography.
- Process scale recovery of biologics
- High flow rates, 100-800 liter/h
- Pharmaceutical standard
- No column-clogging issues
- Automated
- 24/7 operation
- Captures 1-40 liters of MAGicBeads per cycle
- Process 10-4,000 liters of culture volumes
- Recover kgs of mAbs per cycle
PROCESS PARAMETERS
| Product No. | Bead capacity [liter] | Flow rate [l/h] | Feedstock volume [l] | |
|---|---|---|---|---|
| PROCESS Compact | 3100 | 2 | 100 | 10-100 |
| PROCESS Medio | 3300 | 12 | 400 | 60-600 |
| PROCESS Maxi | 3400 | 40 | 800 | 200-4,000 |
Intrested in magnetic separators for process scale? Please contact us.
DATA
Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL：029-851-9222 FAX：029-851-9220
