Product Introduction

Western blot is one of the most prominent lab technique used across various research fields by researchers and educators alike. While most labs still use skim milk as the blocking buffer, it requires extensive amounts of time between incubation and often still results in weak signals even after long exposure times. A lot of time and valuable protein often goes to waste in the process of optimizing for a clearer signal.

✓ Stronger Signal          ✓ Protein-Free                 ✓ Less Antibodies Required

✓ Faster Results             ✓ Competitive Price       ✓ One Solution for All Steps

IE NEO is a next generation western blot blocking & enhancer solution that attempts to solve these problems. With IE NEO’s state-of-the-art nanoparticle composition, it can significantly shorten the incubation time per experiment and achieve clearer signals & reduced background while remaining completely protein-free.

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Feature 1 – Unparalleled Signal Enhancing

IE NEO is designed to enhance the signals even at low protein concentrations. This coupled with the IE NEO’s special blocking properties of the PVDF/NC membranes allow for significantly less background and stronger signals compared to skim milk and even other commercially available western blot enhancers. Below are some experimental data that outlines this property of IE NEO when compared with other blocking solutions under low protein concentrations.

Feature 2 – Less Experimental Steps

With traditional methods of western blot, it is common to have to incubate membranes overnight in skim milk and come back the next day to finish up with the experiment. With IE NEO greatly reducing the amount of steps required before imaging, western blot can easily be done in an afternoon’s time. Additionally, IE NEO is very easy to handle with much less steps required.

Traditional Protocol	NEO Protocol
Tedious Multi-Step Protocol	Simplified Protocol for Easy Handling
Weak Signals at Low Protein Concentration	Strong Signals even at Low Protein Concentrations
Requires Long Incubation Times	Done Within 2.5 hours
Protocol Comparison
Proceed with the regular western blot protocol until completing the PVDF / NC membrane transfer step.
Block with Skim Milk for 1 hr to Overnight	Immerse 1-3min in PBS
Wash 3 times (10min/wash) with PBST/TBST	★Incubate with 1° & 2° Ab diluted in IE NEO for 1-2 hr at RT
Incubate with 1° Ab in appropriate buffer for 1-2 hr at RT
Wash 3 times (10min/wash) with PBST/TBST
Incubate with 2° Ab in appropriate buffer for 1-2 hr at RT
Wash 3 times (10min/wash) with PBST/TBST
Continue with the desired image development methods (ECL / NBT/ etc.)

★IE NEO allows the primary and secondary antibody to be incubated together in a single incubation step. This greatly simplifies the experimental procedures, making it much easier to handle and much faster to get results back.

Feature 3 – Applicable with Most Antibodies / ECL Methods

IE NEO is continuously tested for new antibody combinations, some of the noteworthy combinations are listed below. Additionally IE NEO has been tested to be effective for many ECL methods such as HRP and AP systems, making IE NEO truly an universal western blot blocking & enhancing solution.

Tested Antibodies       (by Antigen)	Host	Type		Tested Antibodies       (by Antigen)	Host	Type
β-actin	Mouse	Monoclonal		STAU2	Rabbit	Polyclonal
AKT	Rabbit	Polyclonal		HSP40/Hdj1	Rabbit	Polyclonal
Vimentin	Goat	Polyclonal		Calreticulin	Rabbit	Polyclonal
Cyclin D1	Rabbit	Polyclonal		p27 Kip1	Rabbit	Polyclonal
PDI	Mouse	Monoclonal		TIA-1/TIAR	Rabbit	Polyclonal
α-Tubulin	Rabbit	Polyclonal		GSK3-β	Rabbit	Polyclonal
sam68	Rabbit	Polyclonal		GAPDH	Rabbit	Polyclonal
G3BP-1	Rabbit	Polyclonal		Hsp90	Rabbit	Polyclonal
TRAF-6	Rabbit	Polyclonal

Product Information

Product Name	Catalog #	Volume	Price	Spec Sheet
IE NEO Western Blot Blocking& Enhancer Solution	IENEO-50	50mL	By inquiry	In preparation
	IENEO-250	250mL	By inquiry	In preparation
	IENEO-500	500mL	By inquiry	In preparation

Please also inquire for bulk volume.