Icosagen

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Icosagen (Estonia) is a research-driven contract research provider for biopharmaceutical and biotechnology industries that deftly adapts to market forces and current trends, and is always guided by the needs of its customers. A portfolio of off-the-shelf products, such as antibodies, proteins, ELISA kits, etc., and quality control services in the field of natural rubber allergen testing and food quality control, are also part of the activities of Icosagen AS.

Product News

Icosagen has released 5 new protein products for SARS-CoV-2 variant strains
P-314-100 SARS-CoV-2 Spike RBD2 South Africa VOC 501.V2 South Africa Variant
P-315-100 SARS-CoV-2 Spike RBD2 Brazil P1 Brazil Variant
P-316-100 SARS-CoV-2 Trimeric Spike South Africa VOC 501.V2 South Africa Variant Stable Trimer
P-317-100 SARS-CoV-2 Trimeric Spike UK VOC 202012/01 UK Variant Stable Trimer
P-318-100 SARS-CoV-2 Trimeric Spike Brazil P1 Brazil Variant Stable Trimer
Icosagen has released 3 new protein products for SARS-CoV-2 Research
P-309-100 SARS-CoV-2 Trimeric Spike Stable Trimer
P-310-100 SARS-CoV-2 Spike S1 UK VOC 202012/01 UK Variant
P-311-100 SARS-CoV-2 Spike RBD2 UK VOC 202012/01 UK Variant

Technology

HybriFree Technology

The proprietary HybriFree method is used for the discovery of monoclonal antibodies from rabbits and chickens. This includes the capture of antibody-producing cells, cloning of VH/VL sequences and construction of human IgG minilibraries for screening. The antibody expression vectors constructed during antibody discovery can be directly used for the manufacturing in Chinese hamster ovary (CHO) cells.

HybriFree Technology

Benefits of the HybriFree Technology

  • Immunization of wild type rabbits and chickens provides great diversity with broad epitope coverage, and picomolar affinities without affinity maturation
  • HybriFree in vivo B cell approach captures antibodies with optimal biochemical properties leading to good developability
  • Good manufacturability of the antibodies is achieved by using CHO cells for the expression of IgG minilibraries that are developed directly from B cells
  • Greater evolutionary distance of chicken yields greater immunogenicity against conserved targets compared to mice
  • HybriFree antibody discovery platform is further powered by Icosagen’s patented QMCF expression technology and extensive biochemical competence resulting in high quality antigen production
  • Icosagen has a proprietary VLP-technology for high level expression of membrane proteins including GPCRs

Benefits of choosing rabbit or chicken for immunization

Mouse
Slow immunization
Normal affinity
Industry standard
Chicken
Fastest immunization
High affinity
The best for conserved targets
Rabbit
Fast immunization
Highest affinity
The best for small molecules

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QMCF Technology

QMCF technology for recombinant protein production is based on replication and stable maintenance of expression vectors in CHO cells, in conjunction with specifically optimized culture conditions. Fast and cost-effective production of recombinant proteins is accomplished by engineering CHO cells to express two viral factors:

  • Polyomavirus Large T antigen (LT)
  • Epstein-Barr Virus EBNA1 protein

Benefits to the clients

  • Recombinant protein production (up to 50 L scale) is managed by PhD-level scientists that guarantee smooth flow of projects (including difficult to express proteins) and transparent communication with clients, supported by detailed project reports
  • Cloning of DNA expression vectors are done with rapid one-step ligase independent method, which is based on Gibson assembly
  • Patented QMCF expression technology in CHO and HEK293 cells provides cost-effectiveness and scalability
  • QMCF CHO cell pools and production cell banks for the commercial supply of diagnostic antibodies can be generated in 10 days
  • Wide range methods for QC analysis are available, including protein concentration, Western blot, PAGE, LabChip, IEF (to determine isoelectric point), analytical SEC (to determine aggregation level), LAL test (to determine endotoxin levels), Octet system (to determine affinity and kinetics), FLOW analysis, DLS (to determine particle size), HILIC HPLC (to determine afucosylation level), LC-MS and LC-MS/MS (to determine proteomic properties)
  • Capabilities to develop methods for the purification of native proteins without tags

Icosagen’s protein production service is based on the QMCF technology that is a robust mammalian expression system for recombinant proteins, delivering up to 1.5 g of antibody per litre of media. Expression vectors are constructed and transfected into CHO cells with our proprietary transfection reagent 007. Proteins are purified from the media with the method that is best suited for the protein of interest. Produced proteins and antibodies have high grade of purity, low endotoxin level and native folding. Deliverables include detailed reports.

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Products

Antibodies



Nucleocapsid Protein SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-350-100 Nucleocapsid protein SARS-CoV-2 Chicken polyclonal IgY
R1-147-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-148-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-149-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal human IgG1-lambda
R1-178-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-kappa
R1-179-100 Nucleocapsid protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-kappa
Spike SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
R1-151-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-156-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-157-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-158-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-159-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-kappa
R1-163-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-164-100 RBD protein SARS-CoV-2 Recombinant human monoclonal Human IgG1-lambda
R1-169-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-170-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
R1-180-100 SARS-CoV-2 virus Recombinant human monoclonal Human IgG1-lambda
BPV Type 1 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A1-100-100 BPV type 1 E2 Mouse monoclonal IgG
A1-101-100 BPV type 1 E2 Mouse monoclonal IgG
A1-150-100 BPV type 1 E2 Mouse monoclonal IgG
A1-151-100 BPV type 1 E2 Mouse monoclonal IgG
A1-152-100 BPV type 1 E2 Mouse monoclonal IgG
A1-153-100 BPV type 1 E2 Mouse monoclonal IgG
A1-154-100 BPV type 1 E2 Mouse monoclonal IgG
A1-155-100 BPV type 1 E2 Mouse monoclonal IgG
Ebolavirus GP Protein100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-100-100 Ebolavirus GP protein Rabbit polyclonal IgG
A2-101-100 Ebolavirus GP protein Monoclonal: rabbit scFv, human Fc hIgG1 scFv-Fc
HPV-18 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-401-100 HPV-18 E2 Mouse monoclonal IgG
HPV-11 E2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-402-100 HPV-11 E2 Mouse monoclonal IgG1
A2-403-100 HPV-11 E2 Mouse monoclonal IgG1
HPV-11 E7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-404-100 HPV-11 E7 Mouse monoclonal IgG2a
A2-405-100 HPV-11 E7 Mouse monoclonal IgG2a
HPV-18 E7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-406-100 HPV-18 E7 Mouse monoclonal IgG1
A2-407-100 HPV-18 E7 Mouse monoclonal IgG1
HCV 1b NS5B100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-420-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-421-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-422-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-423-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-424-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-425-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-426-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-427-100 HCV 1b NS5B Mouse monoclonal IgG1
A2-428-100 HCV 1b NS5B Mouse monoclonal IgG1
C2-424-100 HCV 1b NS5B Partially humanized monoclonal IgG1
HIV-1 Nef100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-801-100 HIV-1 Nef Mouse monoclonal IgG1
A2-802-100 HIV-1 Nef Mouse monoclonal IgG1
A2-803-100 HIV-1 Nef Mouse monoclonal IgG1
A2-804-100 HIV-1 Nef Mouse monoclonal IgG1
A2-806-100 HIV-1 Nef Mouse monoclonal IgG1
A2-807-100 HIV-1 Nef Mouse monoclonal IgG1
A2-809-100 HIV-1 Nef Mouse monoclonal IgG1
A2-811-100 HIV-1 Nef Mouse monoclonal IgG1
HIV-1 Tat100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-821-100 HIV-1 Tat Mouse monoclonal IgG2b
A2-822-100 HIV-1 Tat Mouse monoclonal IgG1
A2-823-100 HIV-1 Tat Mouse monoclonal IgG1
A2-825-100 HIV-1 Tat Mouse monoclonal IgM
A2-826-100 HIV-1 Tat Mouse monoclonal IgG3
A2-827-100 HIV-1 Tat Mouse monoclonal IgG2b
HIV-1 Rev100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-832-100 HIV-1 Rev Mouse monoclonal IgG1
HIV-1 p24100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-851-100 HIV-1 p24 Mouse monoclonal IgG2a
A2-854-100 HIV-1 p24 Mouse monoclonal IgG1
A2-855-100 HIV-1 p24 Mouse monoclonal IgG1
A2-856-100 HIV-1 p24 Mouse monoclonal IgG2b
A2-857-100 HIV-1 p24 Mouse monoclonal IgG2a
A2-858-100 HIV-1 p24 Mouse monoclonal IgG1
A2-859-100 HIV-1 p24 Mouse monoclonal IgG1
C-851-100 HIV-1 p24 Partially humanized monoclonal IgG1
MLV p30100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A1-910-100 MLV p30 Rabbit polyclonal IgG
A2-301-100 MLV p30 Mouse monoclonal IgG
SIV-1 Nef100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-817-100 SIV-1 Nef Mouse monoclonal IgG1
Human GDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
315-100 Human  GDNF Mouse monoclonal murine IgG1
Human Artemin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
323-100 Human Artemin Mouse monoclonal IgG1
324-100 Human Artemin Mouse monoclonal IgG1
325-100 Human Artemin Mouse monoclonal IgG1
326-100 Human Artemin Mouse monoclonal IgG1
Human BDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
327-100 Human BDNF Mouse monoclonal IgG1
328-100 Human BDNF Mouse monoclonal IgG1
329-100 Human BDNF Mouse monoclonal IgG2b
Human CDNF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
300-100 Human CDNF Rabbit polyclonal IgG
301-100 Human CDNF Mouse monoclonal IgG1
302-100 Human CDNF Mouse monoclonal IgG1
Human MANF100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
310-100 Human MANF Rabbit polyclonal IgG
311-100 Human MANF Mouse monoclonal IgG1
341-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
342-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
343-100 Human MANF Recombinant monoclonal antibody mouse IgG2a
Human GFRa-1100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
303-100 Human GFRa-1 Chicken polyclonal IgY
Human GFRa-2100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
304-100 Human GFRa-2 Chicken polyclonal IgY
Human GFRa-3100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
305-100 Human GFRa-3 Chicken polyclonal IgY
Human GFRa-4100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
308-100 Human GFRa-4 Chicken polyclonal IgY
Human Neurturin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
307-100 Human Neurturin Rabbit polyclonal IgG
320-100 Human Neurturin Mouse monoclonal IgG1
321-100 Human Neurturin Mouse monoclonal IgG1
322-100 Human Neurturin Mouse Monoclonal IgG1
Human RET100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
306-100 Human RET Chicken polyclonal IgY
Hev b1100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-700-100 Hev b1 Mouse monoclonal IgG2a
A3-701-100 Hev b1 Mouse monoclonal IgG1
Hev b3100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-704-100 Hev b3 Mouse monoclonal IgG1
A3-705-100 Hev b3 Mouse monoclonal IgG1
Hev b5100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-707-100 Hev b5 Mouse monoclonal IgG1
A3-708-100 Hev b5 Mouse monoclonal IgG2b
Hev b6.02100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A3-709-100 Hev b6.02 Mouse monoclonal IgG1
A3-710-100 Hev b6.02 Mouse monoclonal IgG1
Rabbit Anti-Chicken Polyclonal Antibodies100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
903-100 chicken IgY Rabbit polyclonal, HRP conjugate IgG
904-100 chicken IgY Rabbit polyclonal, DyLight 488 conjugate IgG
905-100 chicken IgY Rabbit polyclonal, DyLight 549 conjugate IgG
Goat Anti-Mouse Polyclonal Antibodies100 μg
Catalogue # Immunogen Clonality Class
A1-900-100 Mouse IgG fraction Goat polyclonal HRP-conjugate IgG
A1-901-100 Mouse IgG fraction Goat polyclonal FITC-conjugate IgG
A1-902-100 Mouse IgG fraction Goat polyclonal, AP-conjugate IgG
Human C-C Motif Chemokine 20 (CCL20)100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
601-100 Human C-C motif chemokine 20 (CCL20) Mouse monoclonal IgG1
Human Elafin100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
630-100 Human Elafin Mouse monoclonal IgG1
Human Interferon Lambda-4 (IFNL4)100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
600-100 Human Interferon lambda-4 (IFNL4) Rabbit polyclonal
Human S100-A8100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
620-100 Human S100-A8 Mouse monoclonal IgG1
Human S100-A9100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
610-100 Human S100-A9 Mouse monoclonal IgG1
Human Cardiac Troponin I100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
102-100 Human cardiac troponin I Mouse monoclonal IgG1
103-100 Human cardiac troponin I Mouse monoclonal IgG1
104-100 Human cardiac troponin I Mouse monoclonal IgG1
105-100 Human cardiac troponin I Mouse monoclonal IgG1
Recombinant Human Cardiac Troponin Complex100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
R1-126-100 Recombinant human cardiac troponin complex Recombinant human monoclonal Human IgG2-lambda
Anti-HEL Human IgG1 Isotype Control1000 μg
Catalogue # Immunogen Clonality Class
R1-146-000 Lysozyme from chicken egg white Recombinant human monoclonal IgG1
His-6 Tag100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-501-100 His-tag Mouse monoclonal IgG2b
Bovine DNase I100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
400-100 Bovine DNase I Rabbit polyclonal IgG
404-100 Bovine DNase I Mouse monoclonal IgG1
405-100 Bovine DNase I Mouse monoclonal IgG1
Human Ribonuclease 7100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
406-100 Human Ribonuclease 7 Mouse monoclonal IgG1
407-100 Human Ribonuclease 7 Mouse monoclonal IgG1
408-100 Human Ribonuclease 7 Mouse monoclonal IgG1
Human Ribonuclease 8100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
430-100 Human Ribonuclease 8 Rabbit polyclonal IgG
Human Laminin a4100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-601-100 Human laminin a4 Mouse monoclonal IgG1
A2-604-100 Human laminin a4 Mouse monoclonal IgG1
A2-607-100 Human laminin a4 Mouse monoclonal IgG1
Human Laminin a5100 μg/500 μg/1000 μg
Catalogue # Immunogen Clonality Class
A2-610-100 Human laminin a5 Mouse monoclonal IgG1

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Proteins

SARS-CoV-2100 μg/500 μg/1000 μg
Catalogue # Product Name Host
P-301-100 SARS-CoV-2 Nucleocapsid-His Protein CHO-based cell line (expressed by QMCF Technology)
P-302-100 Human recombinant ACE2-His Protein CHO-based cell line (expressed by QMCF Technology)
P-303-100 SARS-CoV-2 S1 RBD CHO-based cell line (expressed by QMCF Technology)
P-305-100 SARS-CoV-2 Spike S1 CHO-based cell line (expressed by QMCF Technology)
P-306-100 SARS-CoV-2 Spike S2 ECD-His CHO-based cell line (expressed by QMCF Technology)
P-307-100 SARS-CoV-2 S1 RBD2 CHO-based cell line (expressed by QMCF Technology)
P-308-100 Human recombinant ACE2-hFc CHO-based cell line (expressed by QMCF Technology)
P-309-100 SARS-CoV-2 Trimeric Spike CHO-based cell line (expressed by QMCF Technology)
P-310-100 SARS-CoV-2 Spike S1 UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-311-100 SARS-CoV-2 Spike RBD2 UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-314-100 SARS-CoV-2 Spike RBD2 South Africa VOC 501.V2 CHO-based cell line (expressed by QMCF Technology)
P-315-100 SARS-CoV-2 Spike RBD2 Brazil P1 CHO-based cell line (expressed by QMCF Technology)
P-316-100 SARS-CoV-2 Trimeric Spike South Africa VOC 501.V2 CHO-based cell line (expressed by QMCF Technology)
P-317-100 SARS-CoV-2 Trimeric Spike UK VOC 202012/01 CHO-based cell line (expressed by QMCF Technology)
P-318-100 SARS-CoV-2 Trimeric Spike Brazil P1 CHO-based cell line (expressed by QMCF Technology)
Others10 μg/50 μg/100 μg
Catalogue # Product Name Host
P-100-100 CDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-101-100 MANF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-102-100 Artemin, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-103-100 GDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-104-100 NGF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-105-100 BDNF, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-120-100 GFRA-1-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-121-100 GFRA-2-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-122-100 GFRA-3-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-123-100 GFRA-4-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-124-100 RET-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-200-100 DNase I, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-201-100 DNase I, bovine recombinant CHO-based cell line (expressed by QMCF Technology)
P-300-100 Zaire Ebolavirus envelope glycoprotein Mammalian expression system
P-400-100 Recombinant human RNase 7 CHO-based cell line (expressed by QMCF Technology)
P-402-100 CD48, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-403-100 CD48, mouse recombinant CHO-based cell line (expressed by QMCF Technology)
P-404-100 2B4-His, human recombinant CHO-based cell line (expressed by QMCF Technology)
P-405-100 2B4-His, mouse recombinant CHO-based cell line (expressed by QMCF Technology)
P-500-100 Recombinant murine leukemia virus p30 protein E. coli
P-600-100 Human Cardiac Troponin complex CHO-based cell line (expressed by QMCF Technology)

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ELISA Kits

SARS-CoV-296-well plate
Catalogue # Antibodies Against Detectable Antibodies Substrate
K5-001-096 SARS-CoV-2 IgG, IgM Antigen-coated (S1 and N) microplate wells
Natural Rubber Latex Allergen96-well plate
Catalogue # Product Name Limit of Detection Detection Range Manual MSDS
K3-350-020 FITkit® Hev b 1 ⩽ 2.0 μg/L 10-1000 μg/L Manual MSDS
K3-350-030 FITkit® Hev b 3 ⩽ 2.0 μg/L 10-1000 μg/L Manual MSDS
K3-350-040 FITkit® Hev b 5 ⩽ 1.5 μg/L 5-100 μg/L Manual MSDS
K3-350-010 FITkit® Hev b 6.02 (Hevein) ⩽ 1.5 μg/L 5-200 μg/L Manual MSDS
K3-350-050 ELISA Kit 4 Latex Allergens Hev b 1: 10-1000 µg/L
Hev b 3: 10-1000 µg/L
Hev b 5: 5-100 µg/L
Hev b 6.02: 5-200 µg/L
Manual
Others96-well plate
Catalogue # Product Name Limit of Detection Detection Range Manual MSDS
K2-001-096 Human CDNF ELISA Kit 1-8 pg/mL 15-960 pg/mL Manual
K2-002-096 Human Neurturin ELISA Kit 5-13 pg/mL 50-1600 pg/mL Manual
K2-003-096 Human Artemin ELISA Kit 2-8 pg/mL 30-1920 pg/mL Manual
K2-004-096 Human MANF ELISA Kit 30-75 pg/mL 0.25-16 ng/mL Manual
K4-001-096 Human RNase 7 ELISA kit 1-4 pg/mL 10-640 pg/mL Manual
K4-002-096 Human CD48 ELISA Kit 2-8 pg/mL 25-1600 pg/mL Manual
K4-003-096 Mouse CD48 ELISA Kit 6-16 pg/mL 50-3200 pg/mL Manual

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Transfection Reagents

Catalogue # Description
R007-P001 Reagent 007, 1 mg (powder)
R007-P010 Reagent 007, 10 mg (powder)
TC-010-000 Salmon Sperm Carrier DNA for transfection (10mg/mL) 1mL

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Services

Recombinant Antibody Production

Icosagen offers a broad range of tailored services for the development and production of recombinant antibodies, including complex antibody formats.

Our customers benefit from our cost effective QMCF Expression Technology that is based on the replication, and stable maintenance of the expression vectors in CHO cells, in conjunction with specifically optimized culture conditions for the recombinant antibody production.

There are two options for recombinant antibody production by using QMCF Technology:

  • Enhanced transient production of recombinant antibodies is used to deliver up to hundreds of milligrams of purified antibody within 3-4 weeks starting from vector cloning.
  • Stable episomal production is used to scale up the production volumes up to 40 liters cost effectively, and to deliver multiple gram quantities of purified antibody. In this case, also episomal production cell bank can be generated, which allows to omit transfection completely when repetitive and constant supply of the same antibody is required with consistent reproducibility.

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Antibody Development

Icosagen offers the following antibody development services:

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Protein Production

Icosagen provides recombinant protein production services using proprietary CHO-based QMCF Technology in serum-free conditions. Customer benefits are a short production time and cost-efficiency.

We offer two options for protein production:

  • Enhanced transient expression of recombinant proteins using high copy number transient pQMCF-T expression vectors. This option is fast, and a good fit for a small-scale pilot study to test the best expression conditions for your protein of interest.
  • Production using stable episomal production pools for large-scale production using pQMCF expression vectors that are stably maintained in proliferating CHO cells. This option is scalable and suitable for the cost effective production in up to 40 liter scale, and to produce protein of interest in up to gram-scale.

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Expression of Membrane Proteins

Production of VLP-s pseudotyped with membrane proteins

Icosagen produces VLPs pseudotyped with membrane proteins, such as ion-channels, receptors, viral glycoproteins, etc. For the formation of VLPs, we use retroviral (HIV-1 or MLV) and filoviral (ebola) proteins. VLPs could be produced in 293 or CHO-based QMCF cell lines.

  • Fast and efficient production of VLPs pseudotyped with different receptors, ion-channels, viral glycoproteins, etc.
  • Human-specific posttranslational modification pattern using 293-based cell line

Cell pool generation for membrane protein expression

Icosagen provides services for the fast development of CHO-based cell pools and cell lines, which express membrane proteins. The cell lines could be generated using QMCF Technology in 3 weeks, starting with the cloning of a protein of interest into a QMCF expression vector.

  • Fast and efficient way to generate a stable population of cells expressing a protein of interest; for investigation of signal transduction, screening of active compounds for early stages of drug development
  • Usage of CHO- or U2OS based adherent QMCF cell lines
  • Feasible generation of expression cell banks
  • Assay optimization and validation using Thermo Scientific ArrayScan VTI and appropriate Cellomics software

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Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:029-851-9222 FAX:029-851-9220

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