Fina Biosolutions_Streptavidin Conjugates

Streptavidin Dextrans

Streptavidin-dextran conjugates are soluble dextrans with the streptavidin covalently linked to the polymer. The product is water soluble and has strong binding affinity towards biotin. They are versatile polymers that can be used to increase the valency of biotinylated reagents.

Fina Biosolutions provides Streptavidin-dextrans with dextran backbones of 250 kDa, 500 kDa and 2000 kDa. The conjugates are purified by size exclusion chromatography to remove non-conjugated molecules. Custom ratios & configurations and bulk amounts are available. Please inquire.

A certificate of analysis is provided with each conjugate.

Streptavidin dextran conjugates provided are in sterile PBS at 100µg/ml.

Storage Conditions: Store at 4°C. Protect from light.

Product Concentration Molecular Weight SA/dextran Size
SA-2000kDa dextran low ratio 100 µg/ml 2000 kDa 20 100 ug, 500 ug
SA-500kDa dextran high ratio 100 µg/ml 500 kDa 20 100 ug, 500 ug
SA-500kDa dextran low ratio 100 µg/ml 500 kDa 5 100 ug, 500 ug
SA-250kDa dextran 100 µg/ml 250 kDa ~5 100 ug, 500 ug
Click SA-dextran 100 µg/ml Inquire
Custom SA-dextran products 100 µg/ml Inquire

Fluorescent Streptavidin Dextrans

Fluorescent streptavidin-dextran conjugates are widely known as the gold standard for analyzing qualitative binding between T-cell receptors and T-cell antigens (1-3). Fina Biosolutions offers rPE and APC-conjugated streptavidin-dextrans at competitive prices. Our fluorescent streptavidin-dextran conjugates use our well characterized 500 kDa amino dextran and have been extensively tested with live cells as well as PFA-fixed material. Streptavidin-dextrans with other fluorescent dyes can be prepared as custom products.

Multimers are used to stain T cells for flow cytometry and histology. The name stems from the docking of multiple biotinylated peptide-MHC molecules (pMHCs) onto a backbone. The backbone can be either a single fluorochrome-conjugated streptavidin molecule, which can bind up to 4 pMHCs (“tetramer”) or a fluorochrome-SA-dextran backbone. The highly multivalent presentation of tetramers on a flexible, hydrophilic polymer reveals low-affinity interactions.

fluorescent-streptavidin-dex

References

  1. Dolton et al. Comparison of peptide-major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells. Clin Exp Immunol. 117:47, 2014
  2. Bentzen & Hadrup. Evolution of MHC-based technologies used for detection of antigen-responsive T cells. Cancer Immunol Immunother. 66:657, 2017
  3. Bentzen et al. T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide–MHC complexes. Nature Biotechnology 36:1191, 2018
Product Concentration Molecular Weight Size
Allophycoerythrin/Strepavidin-dextran 100 µg/ml 500 kDa 100 ug
Allophycoerythrin/Strepavidin-dextran 100 µg/ml 2000 kDa 100 ug
rPhycoerythrin/Streptavidin–dextran 100 µg/ml 250 kDa 100 ug
rPhycoerythrin/Streptavidin–dextran 100 µg/ml 500 kDa 100 ug
rPhycoerythrin/Streptavidin–dextran 100 µg/ml 2000 kDa 100 ug
488dye/Strepavidin-dextran 100 µg/ml 250 kDa 100 ug
488dye/Strepavidin-dextran 100 µg/ml 500 kDa 100 ug
488dye/Strepavidin-dextran 100 µg/ml 2000 kDa 100 ug
Dye-streptavidin-dextran (Alexa488, 670 etc) 100 µg/ml Inquire

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Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:029-851-9222 FAX:029-851-9220

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