Fina Biosolutions_Functionalized Dextrans

Overview of Functionalized Dextrans

Fina Biosolutions applies its expertise in carbohydrate chemistry to provide well-characterized and unique dextran products. Products include a wide range of molecular weights and ratios of amino-dextran, monofunctionalized dextrans and multi-functionalized dextrans. We can also provide custom functionalized dextrans and derivatives. See the figure below for some of our dextran products.

functionalizeddextrans
functionalizeddextrans

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Amino-Dextrans

Amino-Dextran (AmDex, AECM-dextran) consists of dextran functionalized with primary amines. (See diagram for the structure of FinaBio amino dextran.) Our AmDex is prepared from dextran using a protocol yielding a stable, amino functionalized dextran that is superior to the more commonly used reductive amination methods. The molecular weight profile of Fina Biosolutions’ AmDex is essentially unchanged from the starting dextran, as determined by analytical size exclusion chromatography. The product is desalted and lyophilized. We do extensive processing and testing to ensure that free amino reagent is removed, resulting in a consistent product with minimal lot-to-lot variation.

We have found that many commercial lots of amino-dextran contain variable amounts of dextran and that the amine content is often incorrect. Furthermore, the actual polymer molecular weight distributions of these preparations can vary extensively from the indicated molecular weight. These issues can mean that optimization data is based on incorrect information. Lot-to-lot variations means that protocols may need to be revised when a new lot of AmDex is used.

Please contact us if you need a ratio that is not listed.

 

* Cat #: (polymer MW) x (amines/polymer)

Cat # Product Size
AD3.5×3 3.5 kDa Amino Dextran 100 mg, 500 mg
AD6x05 06 kDa Amino Dextran 100 mg, 500 mg
AD10x05 10 kDa Amino Dextran 100 mg, 500 mg
AD10x10 10 kDa Amino Dextran 100 mg, 500 mg
AD20x5 20 kDa Amino Dextran 100 mg, 500 mg
AD20x10 20 kDa Amino Dextran 100 mg, 500 mg
AD20x20 20 kDa Amino Dextran 100 mg, 500 mg
AD40x10 40 kDa Amino Dextran 100 mg, 500 mg
AD40x20 40 kDa Amino Dextran 100 mg, 500 mg
AD40x50 40 kDa Amino Dextran 100 mg, 500 mg
AD70x10 70 kDa Amino Dextran 100 mg, 500 mg
AD70x25 70 kDa Amino Dextran 100 mg, 500 mg
AD70x50 70 kDa Amino Dextran 100 mg, 500 mg
AD110x10 110 kDa Amino Dextran 100 mg, 500 mg
AD110x25 110 kDa Amino Dextran 100 mg, 500 mg
AD110x50 110 kDa Amino Dextran 100 mg, 500 mg
AD110x100 110 kDa Amino Dextran 100 mg, 500 mg
AD150x25 150 kDa Amino Dextran 100 mg, 500 mg
AD150x50 150 kDa Amino Dextran 100 mg, 500 mg
AD150x100 150 kDa Amino Dextran 100 mg, 500 mg
AD250x10 250 kDa Amino Dextran 100 mg, 500 mg
AD250x25 250 kDa Amino Dextran 100 mg, 500 mg
AD250x50 250 kDa Amino Dextran 100 mg, 500 mg
AD250x100 250 kDa Amino Dextran 100 mg, 500 mg
AD250x150 250 kDa Amino Dextran 100 mg, 500 mg
AD500x25 500 kDa Amino Dextran 100 mg, 500 mg
AD500x50 500 kDa Amino Dextran 100 mg, 500 mg
AD500x100 500 kDa Amino Dextran 100 mg, 500 mg
AD500x250 500 kDa Amino Dextran 100 mg, 500 mg
AD500x400 500 kDa Amino Dextran 100 mg, 500 mg
AD2000x100 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x250 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x500 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x750 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x1000 2000 kDa Amino Dextran 100 mg, 500 mg

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Amino-Ficoll

As a T-cell independent carrier (TI carrier), coupling Ficoll with haptens has been shown to produce robust antigen-specific primary immune memory1. Our method for amino functionalization yields stable primary amine groups. We carefully control the amine:polymer ratio and process to remove excess amine reagent. Custom Ficoll preparations are available upon request. GE Healthcare’s trademark Ficoll is a highly branched, 400 kDa, hydrophilic polysaccharide widely used for blood separation.

Reference

  1. Inman, JK, Thymus-independent antigens: The preparation of covalent, hapten-Ficoll conjugates. J Imm. 114:705, 1975.
Cat # Product Size
AFic-400×100 100 Amines/400kDa Ficoll 100 mg, 500 mg
AFic-400×150 150 Amines/400kDa Ficoll 100 mg, 500 mg
AFic-400×300 300 Amines/400kDa Ficoll 100 mg, 500 mg

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Biotin-Dextrans

Product Molecular Weight Size
Biotin-dextran 10 kDa 25 mg, 100 mg
Biotin-dextran 40 kDa 25 mg, 100 mg
Biotin-dextran 70 kDa 25 mg, 100 mg
Biotin-dextran 100 kDa 25 mg, 100 mg
Biotin-dextran 250 kDa 25 mg, 100 mg
Biotin-dextran 500 kDa 25 mg, 100 mg
Biotin-dextran 2000 kDa 25 mg, 100 mg
Biotin-amino dextran 10 kDa 25 mg, 100 mg
Biotin-amino dextran 40 kDa 25 mg, 100 mg
Biotin-amino dextran 70 kDa 25 mg, 100 mg
Biotin-amino dextran 100 kDa 25 mg, 100 mg
Biotin-amino dextran 250 kDa 25 mg, 100 mg
Biotin-amino dextran 500 kDa 25 mg, 100 mg
Biotin-amino dextran 2000 kDa 25 mg, 100 mg
Biotin-Fluorescein dextran 10 kDa 25 mg
Biotin-Fluorescein dextran 40 kDa 25 mg
Biotin-Fluorescein dextran 70 kDa 25 mg
Biotin-Fluorescein dextran 100 kDa 25 mg
Biotin-Fluorescein dextran 250 kDa 25 mg
Biotin-Fluorescein dextran 500 kDa 25 mg
Biotin-Fluorescein dextran 2000 kDa 25 mg
Biotin-TMR-dextran 10 kDa 25 mg
Biotin-TMR-dextran 40 kDa 25 mg
Biotin-TMR-dextran 70 kDa 25 mg
Biotin-TMR-dextran 100 kDa 25 mg
Biotin-TMR-dextran 250 kDa 25 mg
Biotin-TMR-dextran 500 kDa 25 mg
Biotin-TMR-dextran 2000 kDa 25 mg

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Fluorescent Dextrans

Fluorescent dextrans have a wide variety of applications. The high water solubility, biocompatibility and range of functionalities make fluorescent dextrans ideal for many applications. They come in an array of molecular weights, fluorescent dyes, dye ratios and additional functional groups. Fluorescent dextrans from Fina Biosolutions use our well-characterized amino dextrans as their base.

This section also lists unique monofluorescent dextrans, which use our proprietary end-labeling chemistry to tag the polymer end with a single fluorescent group.

Fluorescent amino dextrans, also known as “fixable”, contain amino groups which can be reacted with crosslinking reagents such as glutaraldehyde. Fluorescent dextrans do not have any residual free amine groups.

Dextrans with other fluorescent dyes available as custom products.

Please contact us for custom dextran reagents.

 

* TMR: Tetramethylrhodamine-labeled

Product Molecular Weight Form Size
Fluorescein-Amino Dextran 10 kDa 25 mg
Fluorescein-Amino Dextran 20 kDa 25 mg
Fluorescein-Amino Dextran 40 kDa 25 mg
Fluorescein-Amino Dextran 70 kDa 25 mg
Fluorescein-Amino Dextran 250 kDa 25 mg
Fluorescein-Amino Dextran 500 kDa 25 mg
Fluorescein-Amino Dextran 2000 kDa 25 mg
TMR-Amino Dextran 10 kDa 25 mg
TMR-Amino Dextran 20 kDa 25 mg
TMR-Amino Dextran 40 kDa 25 mg
TMR-Amino Dextran 70 kDa 25 mg
TMR-Amino Dextran 250 kDa 25 mg
TMR-Amino Dextran 500 kDa 25 mg
TMR-Amino Dextran 2000 kDa 25 mg
Fluorescein-Dextran 10 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 20 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 40 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 70 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 250 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 500 kDa lyophilized 25 mg, 100 mg
Fluorescein-Dextran 2000 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 10 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 20 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 40 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 70 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 250 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 500 kDa lyophilized 25 mg, 100 mg
TMR-Dextran 2000 kDa lyophilized 25 mg, 100 mg
Mono Fluorescein-Dextran 1.5 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 3.5 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 6 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 10 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 40 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 70 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 250 kDa lyophilized 2 mg, 25 mg
Mono Fluorescein-Dextran 500 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 1.5 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 3.5 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 6 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 10 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 40 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 70 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 250 kDa lyophilized 2 mg, 25 mg
Mono TMR-Dextran 500 kDa lyophilized 2 mg, 25 mg

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Mono-Functionalized Dextrans

Monoamino dextrans and monocarboxyl dextrans are prepared by proprietary methods yielding a stable, monofunctionalized dextran, with a single functional group on the end of the polymer. The molecular weight profile is essentially unchanged, as determined by analytical size exclusion chromatography. Extensive processing and testing is performed to ensure that nonpolymer reagent is removed. This approach results in a consistent product with minimal lot-to-lot variation.

These monofunctionalized dextrans can be used much like PEG—it can be used to increase protein half-life, reduce immunogenicity, and passivate surfaces. They can be labeled with a single fluorescent group to make a well-defined tracer.

Monofluorescent dextrans are listed with fluorescent dextrans.

Monothiol dextran synthesized with a single protected thiol (dithiolpyridyl group) and mono “click” dextrans are available as custom products.

Product Molecular Weight Size
Monoamino Dextran 10 kDa 100 mg, 500 mg
Monoamino Dextran 40 kDa 100 mg, 500 mg
Monoamino Dextran 70 kDa 100 mg, 500 mg
Monoamino Dextran 100 kDa 100 mg, 500 mg
Monoamino Dextran 250 kDa 100 mg, 500 mg
Monoamino Dextran 500 kDa 100 mg, 500 mg
Monocarboxyl Dextran 10 kDa 100 mg, 500 mg
Monocarboxyl Dextran 40 kDa 100 mg, 500 mg
Monocarboxyl Dextran 70 kDa 100 mg, 500 mg
Monocarboxyl Dextran 100 kDa 100 mg, 500 mg
Monocarboxyl Dextran 250 kDa 100 mg, 500 mg
Monocarboxyl Dextran 500 kDa 100 mg, 500 mg
Mono Biotin Dextran 10 kDa 2 mg, 25 mg
Mono Biotin Dextran 40 kDa 2 mg, 25 mg
Mono Biotin Dextran 70 kDa 2 mg, 25 mg
Mono Biotin Dextran 250 kDa 2 mg, 25 mg
Mono Biotin Dextran 500 kDa 2 mg, 25 mg
Monothiol Dextran (Lyophilized) Custom Specifiy MW 25 mg, 100 mg, 500 mg

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Bis-Functionalized Dextrans

Di-amino and Di-carboxy dextrans are prepared by proprietary methods that yield a primary amine or carboxyl group at each end of the dextran polymer. The molecular weight profile is essentially unchanged, as determined by analytical size exclusion chromatography. Extensive processing and testing is performed to ensure that nonpolymer reagent is removed. This approach results in a consistent product with minimal lot-to-lot variation.

These dextrans are unique to Fina Biosolutions and complement our monofunctionalized dextran polymers.

bisfunctionalized-dex

Product Molecular Weight Size
Di-Amino Dextran 6 kDa 100 mg, 500 mg
Di-Amino Dextran 10 kDa 100 mg, 500 mg
Di-Amino Dextran 20 kDa 100 mg, 500 mg
Di-Amino Dextran 40 kDa 100 mg, 500 mg
Di-Amino Dextran 70 kDa 100 mg, 500 mg
Di-Amino Dextran 110 kDa 100 mg, 500 mg
Di-Amino Dextran 250 kDa 100 mg, 500 mg
Di-Amino Dextran 500 kDa 100 mg, 500 mg
Di-Carboxy Dextran 6 kDa 100 mg, 500 mg
Di-Carboxy Dextran 10 kDa 100 mg, 500 mg
Di-Carboxy Dextran 20 kDa 100 mg, 500 mg
Di-Carboxy Dextran 40 kDa 100 mg, 500 mg
Di-Carboxy Dextran 70 kDa 100 mg, 500 mg
Di-Carboxy Dextran 110 kDa 100 mg, 500 mg
Di-Carboxy Dextran 250 kDa 100 mg, 500 mg
Di-Carboxy Dextran 500 kDa 100 mg, 500 mg
Di-Fluorescein Dextran Custom Custom
Di-TAMRA Dextran Custom Custom

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Multi-Functionalized Dextrans

We can provide multi-functionalized dextrans as custom products. These products are based on our proprietary chemistry for end-labeling dextrans.

As there are many combinations, most of our multi-functionalized dextrans are offered as custom products. Please inquire. We appreciate your suggestions for useful new products.

functionalizeddextrans

Mono functionalized- amino dextran

Examples: amino dextran containing a single carboxyl, thiol, biotin, squarate ester, click reagent, etc.

Mono functionalized- carboxy dextran

Examples: carboxy dextran containing a single amino, thiol, biotin, squamate ester, click reagent, etc.

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High Ratio Maleimide Dextrans

functionalizeddextrans

These products are synthesized using a different chemistry than our other modified dextrans. For these products, the maleimide is attached to the dextran via an ester linkage rather than an ether. Dextran functionalized with maleimido moieties is a very useful “ene” compound for thiol-Michael and radical thiol-ene reaction. 6 and 70 kDa maleimide dextrans are available at 0.2 and 1.2 maleimide/glucose (DS). Other molecular weights are available as custom synthesis.

Cat # Product Molecular Weight Maleimide/Glucose Size
MAL-DEX6-0.2 Dextran maleimidohexanoate 6 kDa 0.2 5 mg, 25 mg, 100 mg
MAL-DEX6-1.2 Dextran maleimidohexanoate 6 kDa 1.2 5 mg, 25 mg, 100 mg
MAL-DEX70-0.2 Dextran maleimidohexanoate 70 kDa 0.2 5 mg, 25 mg, 100 mg
MAL-DEX70-1.2 Dextran maleimidohexanoate 70 kDa 1.2 5 mg, 25 mg, 100 mg

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Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:029-851-9222 FAX:029-851-9220

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