Beijing SeekGene BioSciences
Products and Technology
SeekOne™ DD (Digital Droplet) Single Cell 3’Transcriptome kit
SeekOne™ DD (Digital Droplet) Single Cell 3′ Transcriptome-seq is a high-throughput single-cell RNA-seq library kit achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The kit needs to be used with the SeekOne™ Digital Droplet System (SeekOne™ DD) to complete the whole process from single-cell transcriptome barcoding to library construction.
SeekOne™ DD single cell 3′ transcriptome kit includes: SeekOne™ DD Chip S3 (Chip S3 for short), Gasket, Carrier Oil, SeekOne™ DD 3′ Barcoded Beads (Barcoded Beads for short), amplification reagents, library construction reagents, and single cell data analysis software (SeekSoul™ Tools). Based on the principle of microfluidic technology, the kit uses emulsion droplets to separate and capture individual cells, and then recruits nucleic acid-modified Barcoded Beads to molecularly label RNA from different cell sources. Finally, a high-throughput single-cell transcriptome library compatible with Illumina and MGI sequencing system will be constructed, which can be used for research on tumors, immunity, cell development, viral infections, drug discovery, and target screening, etc.
SeekOne™ DD Single Cell Full-length RNA Sequence Transcriptome-seq kit
SeekOne™ Digital Droplet (SeekOne™ DD) High throughput Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit, self- developed by Beijing SeekGene BioSciences Co., Ltd., is a powerful commercial tool for high-throughput whole transcriptome profiling. Compared to conventional 3′ scRNA-seq, scFAST- seq has distinct advantages in detecting non- polyadenylated transcripts, transcript coverage length, and identification of more splice junctions. With target region enrichment, scFAST-seq can simultaneously detect somatic mutations and cell states in individual tumour cells, providing valuable information for precision medicine.
SeekOne™ DD Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq) Kit includes SeekOne™ DD Chip S3 (Chip S3), Gasket, Carrier Oil, SeekOne™ DD scFAST-seq Barcoded Beads, amplification reagents, library construction reagents.
SeekOne™ DD Single Cell Immune Profiling Kit
SeekOne™ DD single-cell immune profiling (5′ transcriptome & immune repertoire sequencing), proposed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The library of both full-length TCR/BCR sequences and mRNA sequences can be constructed from a single sample, followed by high-throughput sequencing and bioinformatics analysis.
The SeekOne DD™ Single Cell Immune Profiling Kits include the SeekOne™ DD Single Cell 5’ Transcriptome-seq Kit and the SeekOne™ DD TCR/BCR amplification Kit. With this kits, the full-length sequence of V(D)J, the pairing information of α chain (heavy chain) and β chain (light chain) of TCR(BCR) and the transcriptome information can be obtained simultaneously. It can be used to investigate T/B cell heterogeneity, TCR/BCR diversity, antigen specificity, vaccine development, immunetherapy screening, etc.
SeekOne™ Digital Droplet System
SeekOne™ Digital Droplet System (SeekOneDD System), self-developed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and capture by water-in-oil droplets generated in the microfluidic system. This provides a one-stop shop for single-cell solutions, from single-cell partitioning, trapping, labelling and library construction to data analysis. It can be applied to a variety of medical studies, including cancer, immunity, cell development, viral infection, drug discovery, and target screening.
SeekMate Tinitan™ Fluoresence Cell Counter
SeekMate TinitanTM FL is a highly automated fluorescence cell counter developed independently by SeekGene Biosciences. It integrates both bright field and fluorescence modes, offering a cutting-edge solution for high-efficiency The dual focusing mode combines automatic and manual focusing, specifically designed for primary cells and special samples. By presetting optimal focusing parameters, it achieves automated accurate counting, overcoming challenges like diverse cell types, varying sizes, irregular shapes, poor compatibility with fluorescent dyes, and interference from impurities and debris. Auto focus Manual focus Fluorescence adjustment One-key call fluorescence cell counting. Equipped with the latest optical components and a dual focusing mode, it captures super clear images. The incorporation of AI deep learning and personalized parameter adjustment provide accurate and stable cell counting solutions for various cell types (including primary cells and cell nuclei). The device is compact and compatible with mobile power sources, making it convenient to carry outdoors for cell counting work in any laboratory setting. SeekMate TinitanTM FL is a handheld “counting expert” with precise counting, full function, flexibility and convenience.
SeekSoul Tools Single-cell Analysis Software
SeekSoul Tools, self-developed by SeekGene is a software for processing and analyzing single-cell transcriptome data. It is used to identify the cell barcode, genome alignment and gene quantification. In this way, a cell expression matrix that can be generated for downstream analysis, followed by cell clustering and expression analysis. The product not only supports the output data of the SeekOne Kits, but also supports various customized design structures through the description of barcode.
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