Arthus Biosystems
We also provide verified SAM and SAH conjugates with tracer molecules such as biotin and digoxin to assist you in developing further research methods. We also provided HRP and fluorescent materials conjugated monoclonal antibodies against SAM and SAH for use conveniently and specifically in a variety of immunoassays for the purposes of quantification and intra-cellular or nuclear localization. The immunofluorescence chromatography test strips provide users with yet other ways to quantify SAM and SAH conveniently and quickly. This method has a few advantages including a better sensitivity, broader detection ranges, quick (in 15 minutes) and broader sample types (serum, plasma and whole blood drop).
The SAM Molecule
A Necessary Chemical
S-Adenosylmethionine (SAM-e, SAMe, SAM, AdoMet, ademetionine) was discovered in Italy by G. L. Cantoni in 1952. It is made from Adenosine Triphosphate (ATP) and Methionone by Methionine Adenosyltransferase. S-adenosylmethionine is a critical metabolite in most living organisms. It performs important functions of transmethylation, transsulfuration, and aminopropylation through decarboxylation.
Since SAM is an intrinsically unstable molecufle, and its optical density maximum of 258-260 nm is not a distinguished absorption, the determination of its concentration in various biological fluids and tissues has always been a challenging task. A simple, convenient method that does not require costly instrumentation (LC, MS, and combinations) is clearly desirable for the determination of the biological concentration of SAM, and to monitor its change and metabolic paths in the body fluids, tissues and organelles.
SAM-e is claimed to be helpful for the following conditions: Depression, Parkinson’s Disease, Osteoarthritis symptoms, Liver disease, Intrahepatic Cholestasis (of pregnancy especially), Fibormyalgia, AIDS-related myelopathy (a spine and nerve disorder associated with AIDS). There has be also limited research that has referenced for SAMe’s role in the following conditions: Attention deficit hyperactivity disorder (ADHD), Premenstrual syndrome (PMS), Premenstrual dysphoric disorder (PMDD), Chronic fatigue syndrome (CFS), heart disease, defective intellectual performance, multiple sclerosis, dementia, Alzeimer’s disease, tendonitis, bursitis, spinal cord injury, seizure, migraine headache, chronic low back pain, lead poisoning and aging.
It is also critically necessary to understand the impact and consequence when SAM is being used daily as a nutritional supplement these days. It has been widely used as over-ther-counter medicine to relieve systems of depression, osteoarthristis and so on.
Methylation
Methylation is involved in nearly every aspect of life. SAM is the sole methyl donor for a variety of methyl-transfer reactions in DNA, RNA, proteins, lipids, phosphilipids, hormones and neurotransmitters. Proper DNA methylation is essential for normal embryonic development. However, when the methyl-transferase gene is homozygously deleted, it is lethal.
DNA improperly methylated has been found in many tumors. Alterations in DNA methylation patterns induce the expression of oncogenes or silence the expression of tumor-suppressor genes. Methyl-deficient diets promote liver cancer in rodents.
Vital Processes
DNA Methylation results in protection of the genome from restriction enzymes. SAM controls gene expression by binding to structural domains embedded within the non-coding region of certain mRNAs. SAM provides the methyl group in the production of essential bio-molecules, including:
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Acetyl-L-Carnitine:
The Neuronutrient & Membrane-Transporting Agent
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Epinephrine & Adrenalin:
The Endogenous Catecholamfine, Stress Hormone, & Neurotransmitter
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Phosphatidylcholine:
The Most Important Membrane Phospholipids
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Carnitine:
The Fat Burner
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Phosphocreatine:
The Primary ATP Reservoir
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Melatonin:
Circadian Rhythm Modulator
SAM is required for cellular growth and repair. It is also involved in the biosynthesis of several hormones and neurotransmitters that affect mood, such as epinephrine. Methyltransferases are also responsible for the addition of methyl groups to the 2′ hydroxyls of the first and second nucleotides next to the 5′ cap in messenger RNA
Transsulfuration
The transsulfuration begins with S-adenosylhomocysteine, or SAH, which is the residual structure of S-adenosylmethionine (SAM) upon transfering methyl group out of SAM. Hydrolysis of SAH yields homocysteine. This converts to cystathionine, cysteine, and finally to glutathione, the hepatocellular antioxidant and life-saving detoxification agent. Since dietary cysteine content is low, and up to 80% of dietary cysteine loses its sulfhydryl groups through the gastrointestinal tract, SAM is the main source of cysteine, the building block of glutathione. As an antioxidant, it protects the body from damaging reactive oxygen molecules in the body. These reactive oxygen molecules can come from inside the body or from environmental pollution and are thought to play a role in the aging process and the development of degenerative disease. In general, SAMe is thought to raise the level of functioning of other amino acids in the body.
Aminopropylation
The aminopropylation is another process initiated with SAM through decarboxylation (decarboxylase). The decarboxylated SAM then couples with putrescine to generate spermidine and spermine which are critical to cell growth, differentiation and the stability of DNA and RNA. Furthermore, Methylthioadenosine (MTA), the by-product of polyamine synthesis, is a powerful analgesic and anti-inflammatory agent. This may be, at least partially, responsible for the clinical benefits observed in the treatment of osteoarthritis, rheumatoid arthritis and fibromyalgia with SAMe.
Product List
Immunoassay Kits and Strips
| Catalog No. | Product Name | Description | Size |
|---|---|---|---|
| IK00201 | SAM ELISA | S-adenosylmethionine ELISA kit | 96 tests |
| IK00201s | SAM ELISA s | S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples | 96 tests |
| IK00202 | SAM ELISA 2 | S-adenosylmethionine ELISA kit, broad range | 96 tests |
| IK00202s | SAM ELISA 2s | S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range | 96 tests |
| IK00203 | SAM ELISA 3 | S-adenosylmethionine ELISA kit, broad range | 48 tests |
| IK00203s | SAM ELISA 3s | S-adenosylmethionine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range | 48 tests |
| IK00204 | SAM ELISA 4 | S-adenosylmethionine ELISA kit for cell culture supernatant or other samples | 96 tests |
| IK00301 | SAH ELISA | S-adenosylhomocysteine ELISA kit | 96 tests |
| IK00301s | SAH ELISA s | S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples | 96 tests |
| IK00302 | SAH ELISA 2 | S-adenosylhomocysteine ELISA kit, broad range | 96 tests |
| IK00302s | SAH ELISA 2s | S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range | 96 tests |
| IK00303 | SAH ELISA 3 | S-adenosylhomocysteine ELISA kit, broad range | 48 tests |
| IK00303s | SAH ELISA 3s | S-adenosylhomocysteine ELISA kit for plasma, serum, or tissue/cell homogenate samples, broad range | 48 tests |
| IK00304 | SAH ELISA 4 | S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples | 96 tests |
| IK00401 | MAT Activity Assay | Methionine Adenosyltransferase (MAT) activity assay kit | 96 tests |
| IK00403 | MAT Activity Assay 3 | Methionine Adenosyltransferase (MAT) activity assay kit | 48 tests |
Antibodies and Conjugates
| Catalog No. | Product Name | Description | Size |
|---|---|---|---|
| MA00201-50 | Mouse anti-SAM 1a | Mouse monoclonal antibody to S-Adenosylmethionine Clone 118-6 | 50 µg |
| MA00201-100 | Mouse anti-SAM 1b | Mouse monoclonal antibody to S-Adenosylmethionine Clone 118-6 | 100 µg |
| MA00202-50 | Mouse anti-SAM 2a | Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-3 | 50 µg |
| MA00202-100 | Mouse anti-SAM 2b | Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-3 | 100 µg |
| MA00203-50 | Mouse anti-SAM 3a | Mouse monoclonal antibody to S-Adenosylmethionine Clone 118-18 | 50 µg |
| MA00204-50 | Mouse anti-SAM 4a | Mouse monoclonal antibody to S-Adenosylmethionine Clone 84-19 | 50 µg |
| PA00201-50 | Rabbit anti-SAM a | Rabbit polyclonal antibody to S-Adenosylmethionine | 50 µg |
| PA00201-100 | Rabbit anti-SAM b | Rabbit polyclonal antibody to S-Adenosylmethionine | 100 µg |
| MA00301-50 | Mouse anti-SAH 1a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-1 | 50 µg |
| MA00302-50 | Mouse anti-SAH 2a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-10 | 50 µg |
| MA00303-50 | Mouse anti-SAH 3a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-3 | 50 µg |
| MA00304-50 | Mouse anti-SAH 4a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 301-16 | 50 µg |
| MA00305-50 | Mouse anti-SAH 5a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-1 | 50 µg |
| MA00306-50 | Mouse anti-SAH 6a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 844-2 | 50 µg |
| MA00307-50 | Mouse anti-SAH 7a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 839-6 | 50 µg |
| MA00308-50 | Mouse anti-SAH 8a | Mouse monoclonal antibody to S-Adenosylhomocysteine Clone 839-12 | 50 µg |
| MAF00201-25 | AF647-anti-SAM 1a | Alexa Fluor® 647 (AF6) conjugated anti-SAM monoclonal antibody Clone 118-6 | 25 µg |
| MAF00201-50 | AF647-anti-SAM 1b | Alexa Fluor® 647 (AF6) conjugated anti-SAM monoclonal antibody Clone 118-6 | 50 µg |
| MAF00202-50 | AF647-anti-SAM 2a | Phycoerythrin (PE) conjugated anti-SAM monoclonal antibody Clone 84-3 | 50 µg |
| MAF00301-25 | AF488-anti-SAH 1a | Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 301-3 | 25 µg |
| MAF00301-50 | AF488-anti-SAH 1b | Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 301-3 | 50 µg |
| MAF00302-50 | AF488-anti-SAH 2a | Alexa Fluor® 488 (AF488) conjugated anti-SAH monoclonal antibody Clone 839-6 | 50 µg |
| MAH00201-50 | HRP-anti-SAM 1a | Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 118-6 | 50 µl |
| MAH00202-50 | HRP-anti-SAM 2a | Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 84-3 | 50 µl |
| MAH00203-50 | HRP-anti-SAM 3a | Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 118-18 | 50 µl |
| MAH00204-50 | HRP-anti-SAM 4a | Horseradish peroxidase (HRP) conjugated anti-SAM monoclonal antibody Clone 84-19 | 50 µl |
| MAH00301-50 | HRP-anti-SAH 1a | Horseradish peroxidase (HRP) conjugated anti-SAH monoclonal antibody Clone 301-3 | 50 µl |
| MAH00302-50 | HRP-anti-SAH 2a | Horseradish peroxidase (HRP) conjugated anti-SAH monoclonal antibody Clone 301-16 | 50 µl |
| MAH00701-50 | HRP-anti-DIG | Peroxidase-conjugated IgG Fraction Monoclonal Mouse Anti-Digoxigenin | 50 µl |
Compounds and Conjugates
| Catalog No. | Product Name | Description | Size |
|---|---|---|---|
| AST00201 | Aza-SAM | A series of S-adenosylmethionine analog Aza-SAM standards: 500nM, 250nM, 125nM, 62.5nM, 31.25nM, 15.625nM, 7.8125nM, 0nM | 1 ml |
| AST00201-1 | Aza-SAM a | S-adenosylmethionine analog Aza-SAM standard. Buffer (4 ml) for serial dilution may be supplied (additional $15 each). | 1 ml (500 nM) |
| AST00201-200 | Aza-SAM b | S-adenosylmethionine analog Aza-SAM standard. Buffer (10 ml) for serial dilution may be supplied (additional $15 each). | 200 µl (5 µM) |
| AST00201-1s | Aza-SAM as | S-adenosylmethionine analog Aza-SAM standard for serum/plasma. Buffer (3.2 ml) for serial dilution may be supplied. | 1 ml (1 µM) |
| ACT00201-5 | PLL-aza-SAM a | Poly-lysine was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). | 5 µg |
| ACT00201-10 | PLL-aza-SAM b | Poly-lysine was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). | 10 µg |
| ACT00202-50 | Bio-SAM a | Biotin is conjugated to NH2 group of S-adenosylmethionine. | 50 µg |
| ACT00202-100 | Bio-SAM b | Biotin is conjugated to NH2 group of S-adenosylmethionine. | 100 µg |
| ACT00203-50 | Bio-6C-SAM 1a | Biotin is conjugated to NH2 of SAM through 6-aminohexanoic acid. | 50 µg |
| ACT00203-100 | Bio-6C-SAM 1b | Biotin is conjugated to NH2 of SAM through 6-aminohexanoic acid. | 100 µg |
| ACT00204-10 | BSA-aza-SAM a | Bovine serum Albumin was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). | 10 µg |
| ACT00204-100 | BSA-aza-SAM b | Bovine serum Albumin was conjugated to S-adenosylmethionine analog Aza-SAM (AST00201). | 100 µg |
| ACT00205-10 | Bio-6C-aza-SAM 1a | Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid. | 10 µg |
| ACT00205-50 | Bio-6C-aza-SAM 1b | Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid. | 50 µg |
| ACT00205-100 | Bio-6C-aza-SAM 1c | Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid. | 100 µg |
| ACT00206-10 | Bio-12CN-aza-SAM 1a | Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker. | 10 µg |
| ACT00206-50 | Bio-12CN-aza-SAM 1b | Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker. | 50 µg |
| ACT00206-100 | Bio-12CN-aza-SAM 1c | Biotin is conjugated to the NH2 of aza-SAM through a 10-carbon and 2-nitrogen linker. | 100 µg |
| AST00301-1 | SAH-Na | S-Adenosylhomocysteine standard (SAH Sodium). Buffer (18 ml) for serial dilution may be supplied (additional $15 each). | 1 ml (4 µM) |
| AST00301-1s | SAH-Na s | S-Adenosylhomocysteine standard (SAH Sodium) for serum/plasma. Buffer (8ml) for serial dilution may be supplied (additional $60 each). | 0.5 ml (4 µM) |
| ACT00301-50 | BSA-SAH | BSA was conjugated to S-Adenosylhomocysteine. | 50 µg |
| ACT00302-50 | Bio-SAH a | Biotin is conjugated to NH2 group of S-adenosylhomocysteine. | 50 µg |
| ACT00302-100 | Bio-SAH b | Biotin is conjugated to NH2 group of S-adenosylhomocysteine. | 100 µg |
| ACT00303-50 | Bio-6C-SAH 1a | Biotin is conjugated to NH2 of SAH through 6-aminohexanoic acid. | 50 µg |
| ACT00303-100 | Bio-6C-SAH 1b | Biotin is conjugated to NH2 of SAH through 6-aminohexanoic acid. | 100 µg |
| ACT00304-25 | Dig-6C-SAH 1a | Digoxin is conjugated to NH2- of SAH through 6-bromocaproic acid | 25 µg |
| ACT00304-50 | Dig-6C-SAH 1b | Digoxin is conjugated to NH2 of SAH through 6-bromocaproic acid. | 50 µg |
| ACT00304-100 | Dig-6C-SAH 1c | Digoxin is conjugated to NH2 of SAH through 6-bromocaproic acid. | 100 µg |
| ACT00305-10 | Bio-12CN-SAH 1a | Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker. | 10 µg |
| ACT00305-50 | Bio-12CN-SAH 1b | Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker. | 50 µg |
| ACT00305-100 | Bio-12CN-SAH 1c | Biotin is conjugated to NH2 of SAH through a 10-carbon and 2-nitrogen linker. | 100 µg |
| AST00401-50 | MAT a | Recombinant methionine adenosyltransferase (E.C. 2.5.1.6), E. Coli expression | 50 µg |
| AST00401-500 | MAT b | Recombinant methionine adenosyltransferase (E.C. 2.5.1.6), E. Coli expression | 500 µg |
| ACT00501-100 | BSA-HCY | Bovine serum albumin (BSA) was conjugated to homocysteine (HCY). | 100 µg |
| AST00601-50 | HMT a | Recombinant Homocysteine S-methyltransferase (E.C. 2.1.1.10), E. Coli expression | 50 µg |
| AST00601-500 | HMT b | Recombinant Homocysteine S-methyltransferase (E.C. 2.1.1.10), E. Coli expression | 500 µg |
Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL：029-851-9222 FAX：029-851-9220
