faCellitate

faCellitate Logo
faCellitate Homepage
faCellitate (Germany) is a venture originated from BASF’s Materials Research, providing interactive consumables for advanced cell technologies and regenerative medicine in the 3D cell culture market. Its unique polymeric platform aims at supporting cancer and stem cell research, drug discovery, toxicology and tissue engineering.

Product List

Catalogue # Product Name Packing CoA
FC0001-96WP BIOFLOAT™ 96-well plates 96-well U-bottom plates CoA

More Info


Technology

Use the innovative faCellitate polymer technology to customize your cell culture. Our unique polymer coatings generate a fully synthetic, biologically relevant cell culture matrix for 3D cell culture, drug discovery, toxicology and stem cell research.

anchor
The polymeric coating easily modifies plastic and glass surfaces without any chemical reactions. The coating contains molecules that rapidly anchor themselves to surfaces through strong physical interactions and self-assembly.
shield
The inert layer prevents the non-specific interaction of proteins and cells at the surface of the substrate. The surface repels biomolecules creating an ultra-low attachment surface – an ideal interface for noise-free biological and biochemical assays.
biotags
Choose any biomolecule to engineer your surface. Our expertise in bioconjugation makes it possible to tailor the presentation and density of ligands and biochemical signals.

Why choose faCellitate coatings?

  • snap
    EASY
    usage
  • FAST
    procedure
  • ROBUST
    coatings
  • DEFINED
    chemistry
  • ADAPTABLE
    to any geometry

BIOFLOAT™ surfaces support reproducible spheroid and organoid culture

RAPID SPHEROID FORMATION

Shorten your experimental timelines. Single spheroids form faster on BIOFLOAT™ surfaces than on most other low attachment or cell-repellent surfaces.

ROUND AND UNIFORM

The 3D cell culture and maintenance on the BIOFLOAT™ surfaces result in highly uniform spheroids and perfect data consistency. Cell satellites and irregular aggregations are eliminated.

RELIABLE SPHEROID CULTURE

The reliable quality of the BIOFLOAT™ surface makes it a premium product for spheroid and organoid culture, including cells which fail to generate spheroids on existing products.

TUNING THE SPHEROID SIZE

The size of the spheroids can be tuned by the number of cells seeded per well

BIOFLOAT™ 96-well plates outperform existing products

  • Highly-defined, cell and protein repelling surface
  • Fully inert and consistent quality
  • Faster generation of spheroids
  • Reproducible data for all kinds of cell lines
  • Elimination of cell satellites and irregular aggregates
  • Outperforming gold standard spheroid and hanging drop plates

Applications are only limited by your scientific imagination

  • Custom icon
    screen compounds in 3D cell culture systems
  • Custom icon
    mimic extracellular environments for stem cells
  • Custom icon
    establish novel and challenging cell cultures
  • Custom icon
    perform noise-free biochemical assays
  • Custom icon
    solve your biointerface challenges

Back to Menu


FAQ

BIOFLOAT™ coating technology provides a highly defined, fully inert cell- and protein-repellent surface for cell culture labware and devices. Compact and reproducible spheroids are rapidly generated on BIOFLOAT™ coated surfaces, even with challenging cell types. Sticky proteins are inhibited to adsorb to your cell culture labware and thereby prevent cell adhesion.

BIOFLOAT™ coating technology is compatible with broad range of human and animal derived cell lines or primary cells. This new technology is developed to generate cell spheroids; therefore, any cell line can be used which is known to form such spheroids, or it can be used to develop new organoid models containing spheroids with cell lines of your choice. In the table below, you can find a selected number of cell lines cultured with BIOFLOAT™ coating technology.

List of Cell Lines
3T3 (Mouse fibroblasts) H1975 (Human Lung adenocarcinoma)
HepG2 (Human hepatoma cell line) MCF-7 (Breast cancer cell line)
Primary Cynomolgus hepatocytes (Monkey) MDA-MB231 (Breast cancer cell lines)
Primary hepatocytes (Human) MCF10A (Breast cancer cell lines)
hDPSC (Primary human dental pulp stem cell line) D492 (Breast epithelial stem cell like cell line)
hDPSC+Panc1 (Human pancreatic cancer cell line) D492HER (Tumorigenic breast epithelial stem cell of D492)
FAMPAC (Human pancreatic adenocarcinoma cell line) RPMI (B-lymphocytes myeloma cell lines)
PRH (Primary rat hepatocytes) Capan-1 (Human pancreatic adenocarcinoma cell line)
PRH+ HHSteC (Human hepatic stellate cells) Mis-Paca (Human pancreatic cell line)
PRH with RHSteC (Rat hepatic stellate cell) HCC1433 (Breast cancer cell line)
H3122 (Human Lung adenocarcinoma) Neuronal stem cells (HN9 differentiated)
H2228 (Human Lung adenocarcinoma) IPSC-Gata6 (induced pluripotent stem cells)

Choose between the ready-to-use 96-well plate or DIY BIOFLOAT™ FLEX coating solution to modify a broad range of plastic and glass surfaces.

The BIOFLOAT™ FLEX coating can be easily used to create a homogeneous and robust coating for your cell culture products, which is stable under standard culture conditions. One pipetting or rinsing step is enough to passivate your surface on the nanometer scale without altering the geometry of your device. The coating solution allows to treat different formats of plates, microfluidic devices or bioreactors, which are amenable for 3D spheroid screening approaches or organoid models in cancer research or toxicology.

The BIOFLOAT™ FLEX coating solution is applicable to a broad range of hydrophobic polymer and glass surfaces such as polystyrene (PS), polycarbonate (PC), polydimethylsiloxane (PDMS), polyethersulfone (PES), cycloolefin copolymer, polyvinyliden fluoride (PVDF), polyethylene (PE) and optical quartz glass.
In order to ensure a high affinity of the coating, the culture surfaces MUST NOT be tissue-culture treated. Cultureware compatible with the BIOFLOAT™ FLEX coating solution are labeled as non-adherent cell culture or not-treated suspension culture products from common suppliers.
Examples can be found below:

BIOFLOAT™ FLEX coating solution instantly creates a monomolecular coating on your surface, which does not modify the surface geometry of your device. Owing to its highly anti-adhesive properties, cell-to-cell interactions are favored, leading to the formation of highly uniform spheroids which float in the medium without interaction with the surface. The coating strongly adheres to the surface and is stable under standard culture conditions including repetitive media exchange.

BIOFLOAT™ FLEX coating solution was designed for culture of eukaryotic cells. All products undergo a quality control to ensure the sterility and for the exclusion of bacterial endotoxins. Therefore, our products are not cell toxic and support the formation of vital cell spheroids.

BIOFLOAT™ FLEX coated well-plates are compatible for test systems using fluorescent light (excitation and emission wavelength 380 nm – 780 nm). Furthermore, with BIOFLOAT™ FLEX also UV-plates can be coated to allow measurements in the range of 260 nm to 280 nm.

Unopened BIOFLOAT™ products can be stored up to one year at room temperature. Storage of opened BIOFLOAT™ products bear the risk of contamination. You can store the opened BIOFLOAT™ FLEX coating solution for four weeks at 4°C. Self-coated as well as our BIOFLOAT™ FLEX ready-to-use well-plates can be stored at 4°C and applied in your experiments as soon as possible to avoid any contamination.

Our BIOFLOAT™ products were developed for cell culture water-based applications. In water-based solutions such as cell culture media our products are very stable. The BIOFLOAT™ FLEX coating solution as well as the coating of the BIOFLOAT™ 96-well plate are not resistant against organic solvent e.g. ethanol or DMSO in high concentrations (>1 %(v/v)).

All BIOFLOAT™ products do not contain any reactive groups, which can react with other chemicals.

BIOFLOAT™ is a nonhazardous good – it contains no substances that are a risk to health. The products are completely biocompatible and non-cell toxic.

BIOFLOAT™ disposable cell culture products can be disposed similar as your standard cell culture plates. After using our products in cell culture, the plates or any other used surfaces are autoclaved and discarded in residual waste.

BIOFLOAT™ FLEX in the delivered bottle as well as BIOFLOAT™ 96-well plates are not autoclavable. The BIOFLOAT™ FLEX coating solution has a boiling temperature in the range of 70°C-100°C. Only BIOFLOAT™FLEX treated devices consisting of the following materials are autoclavable (121°C, 15 psig for 20 minutes): polymethylpentene (PMP), polypropylene (PP) ,polypropylene Copolymer (PPCO), ethylene-chlorotrifluoroethylene copolymer (ECTFE), fluorinated ethylene propylene (FEP), fluorinated ethylene propylene (FEP), polyfluoroalkoxy (PFA), polytetrafluorethylen (PTFE), polysulfone (PSF), polyetherimide (PEI), polyvinylidenfluoride (PVDF), ethylene propylene rubber (EPR).

All products are subjected to sterilization processes by either electron-beam or sterile filtration and subsequently packed into sterile packages. Further sterilization is not necessary.

Our precoated 96-well plates consist of polystyrene (PS) and the BIOFLOAT™ FLEX coating solution is filled into bottles, which consist of polyethylene terephthalate (PETG). Chemical resistances of PS and PETG are listed below.

Chemicals PS PETG
Acids Yes No
Basis (Inorganic) Yes No
Dry salts Yes No
Detergens/surfectans Yes Yes
Aqueous solutions Yes Yes
Media Yes Yes
Back to Menu

Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:029-851-9222 FAX:029-851-9220

一覧へ戻る場合はこちらをクリックして下さい。