ViroStat-Sandwich ELISA Antibody Pairs

Antibody Selection

The benefit of sandwich ELISA (enzyme-linked immunosorbent assay) is its high sensitivity, which is 2-5 times higher than other ELISA such as direct and indirect methods.
On the other hand, it is necessary to select an appropriate antibody combination, and it is not easy to validate the combination from scratch.

When selecting your own sandwich antibody pair, following points should be considered:

1) Using different monoclonal antibodies for capture and detection
— the antibodies should attach to different epitopes, and their locations should not overlap

2) Using same monoclonal antibodies for capture and detection
— multiple identical epitopes should exist for the antibodies to attach

3) Using polyclonal antibody for capture or detection
— check for cross reactivity of the antibodies

In general, using polyclonal antibody for capture and monoclonal antibody for detection will yield a higher sensitivity. Using monoclonal antibody for both capture and detection will yield a higher specificity.

Listed below are the set of validated, best-match antibody pairs for sandwich ELISA available from ViroStat.
Additional information on specificity and cross reactivity are available upon request.


Target Host Capture Antibody Detection Antibody Specificity (Epitope)
Cat. No. Ig Class Cat. No. Ig Class
Influenza A Mouse 1331 IgG1 1371 IgG1 nucleoprotein
Influenza B Mouse 1171 IgG2b 1131 IgG2b nucleoprotein
Mouse 1161 IgG2b 1131 IgG2b nucleoprotein
Respiratory Syncytal Virus Mouse 0631 IgG2a 0635 IgG1 fusion protein
Mouse 0631 IgG2a 0651 IgG2a fusion protein
Group A Streptococcus Rabbit 1511 polyclonal 0435 IgG1 carbohydrate
Goat 1501 polyclonal 0435 IgG1 carbohydrate
Group B Streptococcus Rabbit 1525 IgG1 1525 IgG1 carbohydrate
Adenovirus Mouse 1431 IgG1 1461 IgG1 hexon*1
Mouse 1431 IgG1 1411 IgG2a hexon*1
Metapneumovirus Mouse 4811 IgG1 4821 IgG2a matrix protein
Rotavirus Mouse 0531 IgG2b 0581 IgG2b capsid
Clostridium difficile Mouse 5150 IgG2a 5154 IgG1 toxin A
Mouse 5156 IgG1 5158 IgG1 toxin B
Clostridia GDH Mouse 5186 IgG1 5187 IgG2b GDH
Hepatitis B surface Ag Mouse 1834 IgG2a 1837 IgG2a surface antigen (Ad/Ay)
Mouse 1835 IgG2a 1837 IgG2a surface antigen (Ad/Ay)
Escherichia coli O157 Mouse 1023 IgG2b 1022 IgG1 O157
Mouse 1022 IgG1 1061 IgG3 O157
Campylobacter jejuni Mouse 7721 IgG1 7721 IgG1 100 kDa
Helicobacter pylori Mouse 6641 IgG1 6641 IgG1 54 kDa (flagellin)
Mouse 6641 IgG1 6651 IgG1 54 kDa (flagellin)
Dengue NS1 Mouse 5162 IgG1 5165 IgG1 NS1
Norovirus genogroup II Mouse 4935 IgG1 4933 IgG1 GII
Plasmodium falciparum Mouse 3564 IgG2a 3567 IgG2a HRP2
Salmonella sp. LPS core Mouse 6391 IgG2a 6393 IgG3 common core*2
Mouse 6391 IgG2a 6394 IgG2b common core*2
Listeria sp. Mouse 4219 IgG2b 4217 IgG2b flagella*3
Giardia lamblia Mouse 6281 IgG1 6231 IgG1 65 kDa
Mouse 6271 IgG3 6231 IgG1 65 kDa
Legionella pneumophila Rabbit 6051 polyclonal 6029 IgG3 LPS
Cholera Toxin Mouse 7953 IgG1 7953 IgG1 B subunit
Chlamydia sp. LPS Mouse 1645 IgG2b 1644 IgG2b LPS
Salmonella Typhi Mouse 6345 IgG2a 6345 IgG2a LPS
Herpes simplex 1 & 2 Mouse 0195 IgG1 0197 IgG2a gD
Zika virus Mouse 5122 IgG1 5125 IgG1 NS1
Trichomonas vaginalis Mouse 7141 IgG3 7151 T. vaginalis
Candida albicans Rabbit 6405 IgG1 6405 IgG1 Candida sp.*4
Aspergillus sp. Rabbit 5145 IgG 5145 IgG secreted hyphal antigen*5
Mycobacterium tuberculosis Mouse 5179 IgG 5180 IgG LAM*6


*1) 1431: Known reactivity with 34 serotypes of Adenovirus including types 40 & 41. 1461: Known reactivity with at least 21 serotypes of Adenovirus including types 40 & 41. 1411: Known reactivity with serotypes :1, 2, 3, 4, 5, 6, 7a, 8, 11, 14, 20, 21, 26, 31, 40 & 41.

*2) Positive for all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1, G2. Negative for E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus & Legionella.

*3) Reactive with L. monocytogenes serotypes 1/2a, 1/2b, 3b, 1/2c, 3a, 4a, 4b, 4c, 4d, 4e plus L. innocua, L. ivanovii, L. welshimeri & L. seeligeri. 

*4) Reactive with C. albicans (10 strains), C. parapsillosis, C. glabrata, C. tropicales & C. auris. Negative for C. krusei.

*5) Reactive with 9 Aspergillus species including 6 strains of A. fumigatus.

*6) Reactive with: Mycobacterium tuberculosis (6 strains), M. bovis, M. smegmatis, M. leprae, M. avium, M. phlei & M. parafortuitum.

Please contact us for any inquiries, questions, or information requests.
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