ViroStat-Sandwich ELISA Antibody Pairs|Tokyo Future Style is a bio-tech trading company

Antibody Selection

The benefit of sandwich ELISA (enzyme-linked immunosorbent assay) is its high sensitivity, which is 2-5 times higher than other ELISA such as direct and indirect methods.
On the other hand, it is necessary to select an appropriate antibody combination, and it is not easy to validate the combination from scratch.

When selecting your own sandwich antibody pair, following points should be considered:

1) Using different monoclonal antibodies for capture and detection
— the antibodies should attach to different epitopes, and their locations should not overlap

2) Using same monoclonal antibodies for capture and detection
— multiple identical epitopes should exist for the antibodies to attach

3) Using polyclonal antibody for capture or detection
— check for cross reactivity of the antibodies

In general, using polyclonal antibody for capture and monoclonal antibody for detection will yield a higher sensitivity. Using monoclonal antibody for both capture and detection will yield a higher specificity.

Listed below are the set of validated, best-match antibody pairs for sandwich ELISA available from ViroStat.
Additional information on specificity and cross reactivity are available upon request.

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ViroStat 

Target	Host	Capture Antibody		Detection Antibody		Specificity (Epitope)
		Cat. No.	Ig Class	Cat. No.	Ig Class
Influenza A	Mouse	1331	IgG1	1371	IgG1	nucleoprotein
Influenza B	Mouse	1171	IgG2b	1131	IgG2b	nucleoprotein
	Mouse	1161	IgG2b	1131	IgG2b	nucleoprotein
Respiratory Syncytal Virus	Mouse	0631	IgG2a	0635	IgG1	fusion protein
	Mouse	0631	IgG2a	0651	IgG2a	fusion protein
Group A Streptococcus	Rabbit	1511	polyclonal	0435	IgG1	carbohydrate
	Goat	1501	polyclonal	0435	IgG1	carbohydrate
Group B Streptococcus	Rabbit	1525	IgG1	1525	IgG1	carbohydrate
Adenovirus	Mouse	1431	IgG1	1461	IgG1	hexon*1
	Mouse	1431	IgG1	1411	IgG2a	hexon*1
Metapneumovirus	Mouse	4811	IgG1	4821	IgG2a	matrix protein
Rotavirus	Mouse	0531	IgG2b	0581	IgG2b	capsid
Clostridium difficile	Mouse	5150	IgG2a	5154	IgG1	toxin A
	Mouse	5156	IgG1	5158	IgG1	toxin B
Clostridia GDH	Mouse	5186	IgG1	5187	IgG2b	GDH
Hepatitis B surface Ag	Mouse	1834	IgG2a	1837	IgG2a	surface antigen (Ad/Ay)
	Mouse	1835	IgG2a	1837	IgG2a	surface antigen (Ad/Ay)
Escherichia coli O157	Mouse	1023	IgG2b	1022	IgG1	O157
	Mouse	1022	IgG1	1061	IgG3	O157
Campylobacter jejuni	Mouse	7721	IgG1	7721	IgG1	100 kDa
Helicobacter pylori	Mouse	6641	IgG1	6641	IgG1	54 kDa (flagellin)
	Mouse	6641	IgG1	6651	IgG1	54 kDa (flagellin)
Dengue NS1	Mouse	5162	IgG1	5165	IgG1	NS1
Norovirus genogroup II	Mouse	4935	IgG1	4933	IgG1	GII
Plasmodium falciparum	Mouse	3564	IgG2a	3567	IgG2a	HRP2
Salmonella sp. LPS core	Mouse	6391	IgG2a	6393	IgG3	common core*2
	Mouse	6391	IgG2a	6394	IgG2b	common core*2
Listeria sp.	Mouse	4219	IgG2b	4217	IgG2b	flagella*3
Giardia lamblia	Mouse	6281	IgG1	6231	IgG1	65 kDa
	Mouse	6271	IgG3	6231	IgG1	65 kDa
Legionella pneumophila	Rabbit	6051	polyclonal	6029	IgG3	LPS
Cholera Toxin	Mouse	7953	IgG1	7953	IgG1	B subunit
Chlamydia sp. LPS	Mouse	1645	IgG2b	1644	IgG2b	LPS
Salmonella Typhi	Mouse	6345	IgG2a	6345	IgG2a	LPS
Herpes simplex 1 & 2	Mouse	0195	IgG1	0197	IgG2a	gD
Zika virus	Mouse	5122	IgG1	5125	IgG1	NS1
Trichomonas vaginalis	Mouse	7141	IgG3	7151		T. vaginalis
Candida albicans	Rabbit	6405	IgG1	6405	IgG1	Candida sp.*4
Aspergillus sp.	Rabbit	5145	IgG	5145	IgG	secreted hyphal antigen*5
Mycobacterium tuberculosis	Mouse	5179	IgG	5180	IgG	LAM*6

 

*1) 1431: Known reactivity with 34 serotypes of Adenovirus including types 40 & 41. 1461: Known reactivity with at least 21 serotypes of Adenovirus including types 40 & 41. 1411: Known reactivity with serotypes :1, 2, 3, 4, 5, 6, 7a, 8, 11, 14, 20, 21, 26, 31, 40 & 41.

*2) Positive for all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1, G2. Negative for E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus & Legionella.

*3) Reactive with L. monocytogenes serotypes 1/2a, 1/2b, 3b, 1/2c, 3a, 4a, 4b, 4c, 4d, 4e plus L. innocua, L. ivanovii, L. welshimeri & L. seeligeri. 

*4) Reactive with C. albicans (10 strains), C. parapsillosis, C. glabrata, C. tropicales & C. auris. Negative for C. krusei.

*5) Reactive with 9 Aspergillus species including 6 strains of A. fumigatus.

*6) Reactive with: Mycobacterium tuberculosis (6 strains), M. bovis, M. smegmatis, M. leprae, M. avium, M. phlei & M. parafortuitum.