Fina Biosolutions_Functionalized Dextrans

Overview of Functionalized Dextrans

Fina Biosolutions applies its expertise in carbohydrate chemistry to provide well-characterized and unique dextran products. Products include a wide range of molecular weights and ratios of amino-dextran, monofunctionalized dextrans and multi-functionalized dextrans. We can also provide custom functionalized dextrans and derivatives. See the figure below for some of our dextran products.

functionalizeddextrans
functionalizeddextrans

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Amino-Dextrans

Amino-Dextran (AmDex, AECM-dextran) consists of dextran functionalized with primary amines. (See diagram for the structure of FinaBio amino dextran.) Our AmDex is prepared from dextran using a protocol yielding a stable, amino functionalized dextran that is superior to the more commonly used reductive amination methods. The molecular weight profile of Fina Biosolutions’ AmDex is essentially unchanged from the starting dextran, as determined by analytical size exclusion chromatography. The product is desalted and lyophilized. We do extensive processing and testing to ensure that free amino reagent is removed, resulting in a consistent product with minimal lot-to-lot variation.

We have found that many commercial lots of amino-dextran contain variable amounts of dextran and that the amine content is often incorrect. Furthermore, the actual polymer molecular weight distributions of these preparations can vary extensively from the indicated molecular weight. These issues can mean that optimization data is based on incorrect information. Lot-to-lot variations means that protocols may need to be revised when a new lot of AmDex is used.

Please contact us if you need a ratio that is not listed.

 

* Cat #: (polymer MW) x (amines/polymer)

Cat # Product Size
AD3.5×3 3.5 kDa Amino Dextran 100 mg, 500 mg
AD6x05 06 kDa Amino Dextran 100 mg, 500 mg
AD10x05 10 kDa Amino Dextran 100 mg, 500 mg
AD10x10 10 kDa Amino Dextran 100 mg, 500 mg
AD20x5 20 kDa Amino Dextran 100 mg, 500 mg
AD20x10 20 kDa Amino Dextran 100 mg, 500 mg
AD20x20 20 kDa Amino Dextran 100 mg, 500 mg
AD40x10 40 kDa Amino Dextran 100 mg, 500 mg
AD40x20 40 kDa Amino Dextran 100 mg, 500 mg
AD40x50 40 kDa Amino Dextran 100 mg, 500 mg
AD70x10 70 kDa Amino Dextran 100 mg, 500 mg
AD70x25 70 kDa Amino Dextran 100 mg, 500 mg
AD70x50 70 kDa Amino Dextran 100 mg, 500 mg
AD110x10 110 kDa Amino Dextran 100 mg, 500 mg
AD110x25 110 kDa Amino Dextran 100 mg, 500 mg
AD110x50 110 kDa Amino Dextran 100 mg, 500 mg
AD110x100 110 kDa Amino Dextran 100 mg, 500 mg
AD150x25 150 kDa Amino Dextran 100 mg, 500 mg
AD150x50 150 kDa Amino Dextran 100 mg, 500 mg
AD150x100 150 kDa Amino Dextran 100 mg, 500 mg
AD250x10 250 kDa Amino Dextran 100 mg, 500 mg
AD250x25 250 kDa Amino Dextran 100 mg, 500 mg
AD250x50 250 kDa Amino Dextran 100 mg, 500 mg
AD250x100 250 kDa Amino Dextran 100 mg, 500 mg
AD250x150 250 kDa Amino Dextran 100 mg, 500 mg
AD500x25 500 kDa Amino Dextran 100 mg, 500 mg
AD500x50 500 kDa Amino Dextran 100 mg, 500 mg
AD500x100 500 kDa Amino Dextran 100 mg, 500 mg
AD500x250 500 kDa Amino Dextran 100 mg, 500 mg
AD500x400 500 kDa Amino Dextran 100 mg, 500 mg
AD2000x100 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x250 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x500 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x750 2000 kDa Amino Dextran 100 mg, 500 mg
AD2000x1000 2000 kDa Amino Dextran 100 mg, 500 mg

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Amino-Ficoll

As a T-cell independent carrier (TI carrier), coupling Ficoll with haptens has been shown to produce robust antigen-specific primary immune memory1. Our method for amino functionalization yields stable primary amine groups. We carefully control the amine:polymer ratio and process to remove excess amine reagent. Custom Ficoll preparations are available upon request. GE Healthcare’s trademark Ficoll is a highly branched, 400 kDa, hydrophilic polysaccharide widely used for blood separation.

Reference

  1. Inman, JK, Thymus-independent antigens: The preparation of covalent, hapten-Ficoll conjugates. J Imm. 114:705, 1975.
Cat # Product Size
AFic400x100 100 Amines/400kDa Ficoll 100 mg, 500 mg
AFic400x150 150 Amines/400kDa Ficoll 100 mg, 500 mg
AFic400x300 300 Amines/400kDa Ficoll 100 mg, 500 mg

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Biotin-Dextrans

Product Molecular Weight Size
Biotin-dextran 10 kDa 25 mg, 100 mg
Biotin-dextran 40 kDa 25 mg, 100 mg
Biotin-dextran 70 kDa 25 mg, 100 mg
Biotin-dextran 100 kDa 25 mg, 100 mg
Biotin-dextran 250 kDa 25 mg, 100 mg
Biotin-dextran 500 kDa 25 mg, 100 mg
Biotin-dextran 2000 kDa 25 mg, 100 mg
Biotin-amino dextran 10 kDa 25 mg, 100 mg
Biotin-amino dextran 40 kDa 25 mg, 100 mg
Biotin-amino dextran 70 kDa 25 mg, 100 mg
Biotin-amino dextran 100 kDa 25 mg, 100 mg
Biotin-amino dextran 250 kDa 25 mg, 100 mg
Biotin-amino dextran 500 kDa 25 mg, 100 mg
Biotin-amino dextran 2000 kDa 25 mg, 100 mg
Biotin-Fluorescein dextran 10 kDa 25 mg
Biotin-Fluorescein dextran 40 kDa 25 mg
Biotin-Fluorescein dextran 70 kDa 25 mg
Biotin-Fluorescein dextran 100 kDa 25 mg
Biotin-Fluorescein dextran 250 kDa 25 mg
Biotin-Fluorescein dextran 500 kDa 25 mg
Biotin-Fluorescein dextran 2000 kDa 25 mg
Biotin-TMR-dextran 10 kDa 25 mg
Biotin-TMR-dextran 40 kDa 25 mg
Biotin-TMR-dextran 70 kDa 25 mg
Biotin-TMR-dextran 100 kDa 25 mg
Biotin-TMR-dextran 250 kDa 25 mg
Biotin-TMR-dextran 500 kDa 25 mg
Biotin-TMR-dextran 2000 kDa 25 mg

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Fluorescent Dextrans

Fluorescent dextrans have a wide variety of applications. The high water solubility, biocompatibility and range of functionalities make fluorescent dextrans ideal for many applications. They come in an array of molecular weights, fluorescent dyes, dye ratios and additional functional groups. Fluorescent dextrans from Fina Biosolutions use our well-characterized amino dextrans as their base.

This section also lists unique monofluorescent dextrans, which use our proprietary end-labeling chemistry to tag the polymer end with a single fluorescent group.

Fluorescent amino dextrans, also known as “fixable”, contain amino groups which can be reacted with crosslinking reagents such as glutaraldehyde. Fluorescent dextrans do not have any residual free amine groups.

Dextrans with other fluorescent dyes available as custom products.

Please contact us for custom dextran reagents.

 

* TMR: Tetramethylrhodamine-labeled

Cat. No. Product Molecular Weight Form Size
Flu-Dex10K Fluorescein-Dextran 10 kDa lyophilized 25 mg, 100 mg
Flu-Dex20K Fluorescein-Dextran 20 kDa lyophilized 25 mg, 100 mg
Flu-Dex40K Fluorescein-Dextran 40 kDa lyophilized 25 mg, 100 mg
Flu-dex70K Fluorescein-Dextran 70 kDa lyophilized 25 mg, 100 mg
Flu-Dex250K Fluorescein-Dextran 250 kDa lyophilized 25 mg, 100 mg
Flu-dex500K Fluorescein-Dextran 500 kDa lyophilized 25 mg, 100 mg
Flu-dex2000K Fluorescein-Dextran 2000 kDa lyophilized 25 mg, 100 mg
Flu-Amdex10K Fluorescein-Amino Dextran 10 kDa 25 mg
Flu-Amdex20K Fluorescein-Amino Dextran 20 kDa 25 mg
Flu-Amdex40K Fluorescein-Amino Dextran 40 kDa 25 mg
Flu-Amdex70K Fluorescein-Amino Dextran 70 kDa 25 mg
Flu-Amdex 250K Fluorescein-Amino Dextran 250 kDa 25 mg
Flu-Amdex500K Fluorescein-Amino Dextran 500 kDa 25 mg
Flu-Amdex2000K Fluorescein-Amino Dextran 2000 kDa 25 mg
mFlu-dex1.5K Mono Fluorescein-Dextran 1.5 kDa lyophilized 2 mg, 25 mg
mFlu-dex3.5K Mono Fluorescein-Dextran 3.5 kDa lyophilized 2 mg, 25 mg
mFlu-dex6K Mono Fluorescein-Dextran 6 kDa lyophilized 2 mg, 25 mg
mFlu-dex10K Mono Fluorescein-Dextran 10 kDa lyophilized 2 mg, 25 mg
mFlu-dex40K Mono Fluorescein-Dextran 40 kDa lyophilized 2 mg, 25 mg
mFlu-dex70K Mono Fluorescein-Dextran 70 kDa lyophilized 2 mg, 25 mg
mFlu-Dex250K Mono Fluorescein-Dextran 250 kDa lyophilized 2 mg, 25 mg
mFlu-Dex500K Mono Fluorescein-Dextran 500 kDa lyophilized 2 mg, 25 mg
TMR-dex10K TMR-Dextran 10 kDa lyophilized 25 mg, 100 mg
TMR-dex20K TMR-Dextran 20 kDa lyophilized 25 mg, 100 mg
TMR-dex40K TMR-Dextran 40 kDa lyophilized 25 mg, 100 mg
TMR-dex70K TMR-Dextran 70 kDa lyophilized 25 mg, 100 mg
TMR-dex250K TMR-Dextran 250 kDa lyophilized 25 mg, 100 mg
TMR-dex500K TMR-Dextran 500 kDa lyophilized 25 mg, 100 mg
TMR-dex2000K TMR-Dextran 2000 kDa lyophilized 25 mg, 100 mg
TMR-Amdex10K TMR-Amino Dextran 10 kDa 25 mg
TMR-Amdex20K TMR-Amino Dextran 20 kDa 25 mg
TMR-Amdex40K TMR-Amino Dextran 40 kDa 25 mg
TMR-Amdex70K TMR-Amino Dextran 70 kDa 25 mg
TMR-Amdex250K TMR-Amino Dextran 250 kDa 25 mg
TMR-Amdex500K TMR-Amino Dextran 500 kDa 25 mg
TMR-Amdex2000K TMR-Amino Dextran 2000 kDa 25 mg
mTMR-dex1.5K Mono TMR-Dextran 1.5 kDa lyophilized 2 mg, 25 mg
mTMR-dex3.5K Mono TMR-Dextran 3.5 kDa lyophilized 2 mg, 25 mg
mTMR-dex6K Mono TMR-Dextran 6 kDa lyophilized 2 mg, 25 mg
mTMR-dex10K Mono TMR-Dextran 10 kDa lyophilized 2 mg, 25 mg
mTMR-dex40K Mono TMR-Dextran 40 kDa lyophilized 2 mg, 25 mg
mTMR-dex70K Mono TMR-Dextran 70 kDa lyophilized 2 mg, 25 mg
mTMR-Dex250K Mono TMR-Dextran 250 kDa lyophilized 2 mg, 25 mg
mTMR-Dex500K Mono TMR-Dextran 500 kDa lyophilized 2 mg, 25 mg

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Mono-Functionalized Dextrans

Monoamino dextrans and monocarboxyl dextrans are prepared by proprietary methods yielding a stable, monofunctionalized dextran, with a single functional group on the end of the polymer. The molecular weight profile is essentially unchanged, as determined by analytical size exclusion chromatography. Extensive processing and testing is performed to ensure that nonpolymer reagent is removed. This approach results in a consistent product with minimal lot-to-lot variation.

These monofunctionalized dextrans can be used much like PEG—it can be used to increase protein half-life, reduce immunogenicity, and passivate surfaces. They can be labeled with a single fluorescent group to make a well-defined tracer.

Monofluorescent dextrans are listed with fluorescent dextrans.

Monothiol dextran synthesized with a single protected thiol (dithiolpyridyl group) and mono “click” dextrans are available as custom products.

Cat. No. Product Molecular Weight Size
mA10 Monoamino Dextran 10 kDa 100 mg, 500 mg
mAD40 Monoamino Dextran 40 kDa 100 mg, 500 mg
mAD70 Monoamino Dextran 70 kDa 100 mg, 500 mg
mAD110 Monoamino Dextran 100 kDa 100 mg, 500 mg
mAD250 Monoamino Dextran 250 kDa 100 mg, 500 mg
mAD500 Monoamino Dextran 500 kDa 100 mg, 500 mg
mBiotDex10 Mono Biotin Dextran 10 kDa 2 mg, 25 mg
mBiotDex40 Mono Biotin Dextran 40 kDa 2 mg, 25 mg
mBiotDex70 Mono Biotin Dextran 70 kDa 2 mg, 25 mg
mBiotDex250 Mono Biotin Dextran 250 kDa 2 mg, 25 mg
mBiotDex500 Mono Biotin Dextran 500 kDa 2 mg, 25 mg
mCD10 Monocarboxyl Dextran 10 kDa 100 mg, 500 mg
mCD40 Monocarboxyl Dextran 40 kDa 100 mg, 500 mg
mCD70 Monocarboxyl Dextran 70 kDa 100 mg, 500 mg
mCD100 Monocarboxyl Dextran 100 kDa 100 mg, 500 mg
mCD250 Monocarboxyl Dextran 250 kDa 100 mg, 500 mg
mCD500 Monocarboxyl Dextran 500 kDa 100 mg, 500 mg
Monothiol dextran Inquire 25 mg, 100 mg, 500 mg

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Multi-Functionalized Dextrans

We can provide multi-functionalized dextrans as custom products. These products are based on our proprietary chemistry for end-labeling dextrans.

As there are many combinations, most of our multi-functionalized dextrans are offered as custom products. Please inquire. We appreciate your suggestions for useful new products.

functionalizeddextrans

Mono functionalized- amino dextran

Examples: amino dextran containing a single carboxyl, thiol, biotin, squarate ester, click reagent, etc.

Mono functionalized- carboxy dextran

Examples: carboxy dextran containing a single amino, thiol, biotin, squamate ester, click reagent, etc.

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High Ratio Maleimide Dextrans

functionalizeddextrans

These products are synthesized using a different chemistry than our other modified dextrans. For these products, the maleimide is attached to the dextran via an ester linkage rather than an ether. Dextran functionalized with maleimido moieties is a very useful “ene” compound for thiol-Michael and radical thiol-ene reaction. 6 and 70 kDa maleimide dextrans are available at 0.2 and 1.2 maleimide/glucose (DS). Other molecular weights are available as custom synthesis.

Cat # Product Molecular Weight Maleimide/Glucose Size
MAL-DEX6-0.2 Dextran maleimidohexanoate 6 kDa 0.2 5 mg, 25 mg, 100 mg
MAL-DEX6-1.2 Dextran maleimidohexanoate 6 kDa 1.2 5 mg, 25 mg, 100 mg
MAL-DEX70-0.2 Dextran maleimidohexanoate 70 kDa 0.2 5 mg, 25 mg, 100 mg
MAL-DEX70-1.2 Dextran maleimidohexanoate 70 kDa 1.2 5 mg, 25 mg, 100 mg

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Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL:029-851-9222 FAX:029-851-9220

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