Allergen raw materials – Citeq Biologics
 CITEQ Homepage |
Citeq Biologics（the Netherlands）is an expert in the field of natural allergen production in the field of allergy and asthma. Their expertise lies in the production of natural allergens of insects and moulds for professional use. Their cultivation technique allows them to make highly concentrated allergens, thus resulting in the provision of strong raw materials, whole cultures, extracts, and purified major allergens. |
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Production of allergen source materials
For the production of source material CITEQ use mites as raw materials to prepare various extracts, e.g. from whole cultures or mite bodies, to obtain defined freeze-dried extracts. Both the raw materials as well as the freeze-dried extracts can be used for diagnosis, research and immunotherapy purposes, provided that the consistency is ensured. CITEQ produce under well-defined parameters which are monitored and documented, offering a consistent product quality to meet customer standards.
Controlled production
The production process to obtain an acceptable and an as pure as possible freeze-dried extract as end product from raw materials consists of several steps. First, to extract the allergenic proteins it is important that the appropriate buffers are used in the starting material as well as temperature controlled circumstances during the process. Next, the allergenic proteins which are in the solution are separated from non-soluble substances by means of centrifugation and filtration.
Consistent quality
To ensure extract consistency, source materials are obtained from cultures in a well-defined medium. The culture conditions have been established for each of the mite species to support optimal growth as well as controlled major allergen content.
In an ideal world, allergens would be supplied with a quantified biologic activity. Unfortunately, science cannot define this biological activity. To secure our production process we have defined parameters to set our specifications, which CITEQ monitors and documents.
Major advantages of CITEQ’s freeze dried extracts
- High extract consistency
- Extreme low allergy content of medium
- Direct use of freeze dried extract; production occurs without preservatives or buffer salts
- Defined source material: we deliver our material with extensive information about the amount of allergens in our material so you really can tell what you are using for your application.
- Large batches: we produce in large batches so we can deliver from the same batch over a longer period of time. This means you are assured of the same material and consistency. If you are using our material and you are satisfied with it you can even place a reservation of a specific LOT so you will receive the same material in the future.
- Freeze-dried: our material will be delivered in freeze-dried form (lyophilization) to assure a long shelf life and can easily be used to reach targeted dilutions.
- Available in different sizes such as 10 mg, 100 mg and 1 gram (specific sizes can be delivered on request).
- No animal or human components: our mites are cultivated on defined media without any animal or human components.
House Dust Mites (HDM)
House dust mites are arachnoids and are present in every household and are living within dust, textile, carpets, beds and upholster furniture. HDMs belong to the Pyroglyphidae family. The most important House dust mites are Dermatophagoides pteronyssinus and Dermatophagoides farinae (in drier areas). HDMs contain allergens which is a (non) protein or substance which can induce an allergic response. Humans mostly get exposed to house dust mites and other indoor allergens. Humans who are atopic can react on this exposure by developing an allergy. Allergic responses can result in eczema, conjunctivitis, asthma symptoms and rhinitis.
Our main focus is the production of house dust mites (HDMs) and their allergens. Citeq was born based on a passion for mites and the research for how mites influence our daily lives. Our founders and leading scientists have pharmaceutical, biological and biochemical backgrounds. With over 25 years of experience and an extensive knowledge about mites Citeq is more than just a supplier of allergen source material. We strive for an academic collaboration with our customers in order to achieve the best possible results in research, diagnostics and immunotherapy.
Our specialty starts with our cultivation medium which has an absolute low in foreign protein. Our cultivation technique leads to high concentrations of the major allergens, resulting in strong raw materials, extracts in several grades of purity, and very pure major allergens. All our house dust mite products contain the native proteins, with no denaturing treatments during production and purification
| D. pteronyssinus (HDM) | Amount | |
| 02.01.30 | Mite bodies | 1g |
| 02.01.40 | Semi-purified mite bodies | 1g |
| 02.01.60 | Whole culture | 1g |
| 02.01.64 | Extract with low endotoxin | 10mg / 100mg / 1g |
| 02.01.71 | Der p 1 purified (>95%) | 400-500μg (BCA) |
| 02.01.72 | Der p 2 purified (>95%) | 200-300μg (BCA) |
| 02.01.85 | Extract | 10mg / 100mg / 1g |
| 02.01.86 | Mite bodies extract | 10mg / 100mg / 1g |
| 02.01.87 | Excrements | 250mg / 1g |
| 02.01.95 | Extract (micro vial) filled out on BCA | 200μg (BCA) |
| 02.50.85 | D. pteronyssinus/ D. farinae extract mix | 10mg / 100mg / 1g |
| D. farinae (HDM) | Amount | |
| 02.02.30 | Mite bodies | 1g |
| 02.02.40 | Semi-purified mite bodies | 1g |
| 02.02.60 | Whole culture | 1g |
| 02.02.64 | Extract with low endotoxin | 10mg / 100mg / 1g |
| 02.02.71 | Der f 1 purified (>95%) | 400-500μg (BCA) |
| 02.02.72 | Der f 2 purified (>95%) | 200-300μg (BCA) |
| 02.02.85 | Extract | 10mg / 100mg / 1g |
| 02.02.86 | Mite bodies extract | 10mg / 100mg / 1g |
| 02.02.87 | Excrements | 5mg |
| Euroglyphus maynei (HDM) | Amount | |
| 02.04.60 | Whole culture | 1g |
| 02.04.85 | Extract | 10mg / 100mg / 1g |
| Anti-mouse antibodies | Amount | |
| HE.02.01 | Anti-mouse IgE | 1 vial |
| HE.02.02 | Anti-mouse IgG1 | 1 vial |
| HE.02.03 | Anti-mouse IgG2a | 1 vial |
| ELISA Kits | Amount | |
| 100.01.50 | Der p 1 ELISA kit | 1 kit |
| 100.01.55 | Der p 2 ELISA kit | 1 kit |
| 100.02.50 | Der f 1 ELISA kit | 1 kit |
| 100.05.01 | ELISA plate coating stabilizer | 1 vial / 4 vials |
| 100.05.02 | Antibody reconstitution buffer | 1 vial / 4 vials |
Biotinylated Materials
During biotinylation biotin is attached to macromolecules or proteins. Biotinylated proteins can, due to the high specifity of biotin-avidin interaction, be used to detect and/ or purify proteins. Citeq has developed house dust mite related biotinylated proteins. These biotinylated proteins include D. pteronyssinus (HDM) extract and the natural purified Der p 1 protein and Der p 2 protein. Biotinylated proteins can be applicated in many protein assays. These protein assays include for example western blot analysis, fluorescence-activated cell sorting (FACS)/ flow cytometry and enzyme-linked immunosorbant assay (ELISA).
| Biotinylated products | Amount | |
| 02.01.73 | Biotinylated Der p 1 | 1 vial |
| 02.01.74 | Biotinylated Der p 2 | 1 vial |
| 02.01.88 | Biotinylated D. pteronyssinus | 1 vial |
| 02.02.88 | Biotinylated D. farinae | 1 vial |
Storage Mites
Traditionally Citeq has cultivated 8 species of acarid storage mites (stored product mites) in the families Acaridae, Glycphagidae and Pyroglyphidae. We can deliver the following storage mites: Blomia tropicalis and Acarus Siro.
For research purposes we can also deliver Glycyphagus domesticus, Lepidoglyphus destructor, Tyrophagus putrescentiae, Lepidoglyphus fustifer, Austroglycyphagus geniculatus and Chortoglyphus arcuatus.
Stored product mites are arachnoids and are present in stored food like dried fruits, flour and cereal products. Besides that they can also be found outdoors within hay and straw. The most common species are Blomia tropicalis, Acarus Siro, Glycyphagus domesticus and Lepidoglyphus destructor. Storage mites are a possible cause of allergic disorders and mostly present among agriculture population. Storage mites can also adapt their feeding to house dust. Therefore, they can also cause allergy in more urban areas where the mites life indoor.
| Acarus siro (storage mites) | Amount | |
| 01.01.60 | Whole culture | 1g |
| 01.01.85 | Extract | 10mg / 100mg / 1g |
| Blomia tropicalis (storage mites) | Amount | |
| 01.05.60 | Whole culture | 1g |
| 01.05.85 | Extract | 10mg / 100mg / 1g |
| Glycyphagus domesticus (storage mites) | Amount | |
| 01.03.60 | Whole culture | 1g |
| 01.03.85 | Extract | 10mg / 100mg / 1g |
| L. destructor (storage mites) | Amount | |
| 01.02.60 | Whole culture | 1g |
| 01.02.85 | Extract | 10mg / 100mg / 1g |
| T. putrescentiae (storage mites) | Amount | |
| 01.04.60 | Whole culture | 1g |
| 01.04.85 | Extract | 10mg / 100mg / 1g |
Moulds
Molds belong to the kingdom Fungi. Molds reproduce by releasing “seeds”, called spores, in the air. These spores contain allergens. Inhaling these spores lead, in atopic human, to an allergic reaction. Spores spread mainly from spring to early fall. Due to the fact that fungi also lives indoor, allergic reactions will occur throughout the entire year. It is estimated that 3-10% of the population, worldwide, is sensitized to mold. From all of the mold species only a few can cause allergic reactions. One of these species is the Alternaria alternata (also know as Alternaria tenuis) which is abundantly present indoor and outdoor. A. alternata has allergens which contains both mycelial fragments and airborne spores which can cause allergic symptoms. Humans who suffer from mold allergy have an IgE reactivity roundabout 70% to Alternaria. A mold allergy can cause a severe respiratory reaction to persons with allergic bronchitis/ asthma.
| Alternaria alternata | Amount | |
| 09.01.26 | Medium | 10mg / 100mg / 1g |
| Aspergilus fumigatus | Amount | |
| 09.02.26 | Medium | 10mg / 100mg / 1g |
Cockroach
Cockroaches contain one of the major indoor allergens, inducing respiratory allergies. Cockroaches can be found in large numbers worldwide, this makes cockroaches a cosmopolitan. Worldwide there life about 3500 different cockroach species. The five cockroaches which are known to induce respiratory allergies include Blattella germanica, Blatta orientalis, Periplaneta americana, Supella longipalpis and Periplaneta fuliginosa. Sensitisation to these species in asthma patients varies between 30-60% worldwide. Blattella germanica is a common specie all over the world, therefore, B. germanica is often found in sensitisation profiles of patients with respiratory allergy.
| Blattella germanica | Amount | |
| 05.01.85 | Whole bodies extract | 10mg / 100mg / 1g |
| Blatta orientalies | Amount | |
| 05.02.85 | Whole bodies extract | 10mg / 100mg / 1g |
| Periplaneta americana | Amount | |
| 05.03.85 | Whole bodies extract | 10mg / 100mg / 1g |
Venom
Hymenoptera consist of different families, including the families Apidae and Vespidae. Three species which are very important in the context of venom allergies are Vespula vulgaris (the common wasp), Vespula germanica (german wasp) and Apis mellifera (honey bee). Stings of Hymenoptera occur very frequently. 0.3-7.5% of the population, worldwide, suffer from venom allergy. When humans, with a venom allergy, get stung they will suffer from severe local reactions, systemic symptoms. Atopic humans will suffer from an anaphylactic schock after a sting. In central Europe, 30-40% of the human with a venom allergy have an allergy for both, bee and wasp, venoms.
| Bee venom, Apis mellifera | Amount | |
| 04.07.85 | Extract | 25 mg |
| Wasp venom, V. vulgaris/ V. germanica mix | Amount | |
| 04.10.85 | Extract | 25 mg |
Please contact us for any inquiries, questions, or information requests.
Tokyo Future Style, Inc.
info@tokyofuturestyle.com
TEL：029-851-9222 FAX：029-851-9220
