ACROBiosystems – CD47
BackgroundProduct ListAssay data
Background
CD47,or Integrin-associated protein (IAP), is first discovered in a study aimed at studying integrin αvβ3. It turned out to be associated in cis with integrin αvβ3 on the cell membrane. Followup studies has established CD47 as the quintessential “marker of self” expressed in all cell types. It’s a key regulator of tissue homeostasis, and is also involved in a variety of diseases ranging from atherosclerosis to cancer. CD47 protects the host cells by suppressing phagocytosis. It’s well established that cancer cell-expressed CD47 interacts with the NK cell-expressed SIRP family proteins, SIRP alpha and SIRP gamma. By so doing, it protects the cancer cell from being eliminated by the innate immune response.
In addition, CD47 is also essential for TSP-1 mediated vascular NO signaling. It regulates CD36 and VEGFR2 to inhibit angiogenesis. Long term dysregulation of NO signaling is a hallmark for several types of cardiovascular diseases, which makes CD47 a promissing target for atherosclerosis. Indeed, Anti-CD47 mAb has been demonstrated to reduce the atherosclerotic burden in several in vivo models.
Related Page: Introduction of ACROBiosystems
Related Page: Bispecific antibody targets
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Given its significant role in pathogenesis of cancer and cardiovascular diseases, there is no surprise that CD47 signaling has been heavily investigated for new targeted therapeutics.
ACROBiosystems provides a unique set of CD47-relevant products, including mutilple avi tag pre-biotinylated proteins, for rapid high throughput screening. The following pairs of products can be easily adapted to ELISA, Alphalisa, and MSD platform to screen neutralizing compound. |
Product List
| Cat. No. | Product Description |
|---|---|
| CD7-H5227 | Human CD47 Protein, His Tag Hot |
| CD7-H5256 | Human CD47 Protein, Fc Tag (MALS verified) Hot |
| CD7-H82E9 | Biotinylated Human CD47 Protein, His,Avitag™ Hot |
| CD7-H82F6 | Biotinylated Human CD47 Protein, Fc,Avitag™ Hot |
| CD7-H52A5 | Human CD47 Protein, Mouse IgG2a Fc Tag, low endotoxin Hot |
| EP-102 | CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair Hot |
| CD7-H522a | Human CD47 Protein, His Tag, low endotoxin |
| CD7-H5251 | Human CD47 Protein, Llama IgG2b Fc Tag, low endotoxin |
| CD7-H82A3 | Biotinylated Human CD47 Protein, Mouse IgG2a Fc,Avitag™ |
| CD7-H82F8 | Biotinylated Human CD47 Protein, Fc (L234A, L235A, P329G),Avitag™ |
| CD7-HA2E9 | Unconjugated Human CD47 Protein, His,Avitag™ |
| CD7-HF2H3 | FITC-Labeled Human CD47 Protein, His Tag |
| CD7-M522b | Mouse CD47 Protein, His Tag |
| CD7-M5251 | Mouse CD47 Protein, Fc Tag |
| CD7-M525a | Mouse CD47 Protein, Mouse IgG2a Fc Tag, low endotoxin |
| CD7-M82E4 | Biotinylated Mouse CD47 Protein, His,Avitag™ |
| CD7-R5256 | Rat CD47 Protein, Fc Tag |
| CD7-R5257 | Rabbit CD47 Protein, Fc Tag |
| CD7-C5252 | Cynomolgus / Rhesus macaque CD47 Protein, Fc Tag |
| CD7-C52H1 | Cynomolgus / Rhesus macaque CD47 Protein, His Tag |
| CD7-C52H5 | Canine CD47 Protein, His Tag |
| CDA-H82F2 | Biotinylated Human SIRP alpha / CD172a Protein, Fc,Avitag™ (MALS verified) |
| SIA-H5225 | Human SIRP alpha / CD172a Protein, His Tag (MALS verified) |
| SIA-H5251 | Human SIRP alpha / CD172a Protein, Fc Tag (MALS verified) |
| SIA-H52A8 | Human SIRP alpha / CD172a Protein, Mouse IgG1 Fc Tag (MALS verified) |
| SIA-H82A3 | Biotinylated Human SIRP alpha / CD172a Protein, Mouse IgG2a Fc,Avitag™ |
| SIA-H82E0 | Biotinylated Human SIRP alpha / CD172a Protein, His,Avitag™ (MALS verified) |
| SIA-HP252 | PE-Labeled Human SIRP alpha / CD172a Protein, Fc Tag (recommended for neutralizing assay) |
| SIA-M5252 | Mouse SIRP alpha / CD172a Protein, Mouse IgG2a Fc Tag, low endotoxin |
| SIA-M5258 | Mouse SIRP alpha / CD172a Protein, Fc Tag |
| SIA-M52H4 | Mouse SIRP alpha / CD172a Protein, His Tag |
| SIA-C5254 | Cynomolgus SIRP alpha / CD172a Protein, Fc Tag |
| SIA-C52H7 | Cynomolgus SIRP alpha / CD172a Protein, His Tag |
| SIA-H5257 | Human SIRP beta / CD172b Protein, Fc Tag |
| SIG-H5253 | Human SIRP gamma / CD172g Protein, Fc Tag |
More BsAb targets↓
Assay data
High homogeneity verified by HPLC
The purity of Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) was greater than 90% as determined by SEC-HPLC.
Verification of CD47 binding to SIRP-α
| Bioactivity-ELISA: CD47 can bind to SIRP-α | Bioactivity-BLI: CD47 can bind to SIRP-α |
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| Immobilized Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) with a linear range of 8-250 ng/mL. | Immobilized Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) |
| Cell based assay: CD47 can bind to ACHN cell expressing SIRP-α | |
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| FACS assay shows that Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) can bind to ACHN cell expressing SIRP-α. The concentration of CD47 used is 10 μg/mL. |
Application of CD47 antibody screening
Antibody affinity testing
| Bioactivity-ELISA: CD47 can bind to Anti-CD47 MAb | |
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| Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, His,Avitag (Cat. No. CD7-H82E9) with a linear range of 0.05-2 ng/mL | |
| Bioactivity-SPR/BLI: CD47 can bind to Anti-CD47 MAb | |
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| Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) | Immobilized Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) |
Application of neutralizing antibody screening
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| Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 124.5 ng/mL. | FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody with the IC50 15.02 μg/mL. The concentration of Human CD47 used is 10 μg/mL. |
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